Notebook

06.07.09

Wet lab

LOVTAP plasmid AND TrpR plasmid were transformed in competent E. Coli, and grown overnight.
One problem: we actually don't know TrpR plasmid resistance, so we tried with three resistances available in the lab: Amp., Kana. and Chl. LOVTAP is in a plasmid called pCal-n (see picture below):

pCal-n plasmid

Some comments on the plasmid: - CBP is a small peptide with which we could purify LOVTAP protein -

Cloning strategy

Four primers were designed to amplify:

1. Promoter T7, RBS, CBP and LOVTAP
2. RBS, CBP and LOVTAP
3. CBP and LOVTAP
4. LOVTAP