Team:EPF-Lausanne/Safety

Introduction

What is biosafety ?

Biosafety is a group of rules that regulates the way genetically modified organisms or pathogen agents have to be manipulated. The aim is to protect people, their surroundings and environment. There five types of safety rules, that each have specific types of measure : technical (containment, security place, ...), organizational (procedures, responsabilities, ...), administrative (authorizations, notifications, ...), personal (formation, protection equipment, ...), and monitoring (analysis, dissemination controls, ...).

At the beginning of iGEM project, we all received a little formation on how behaving in a lab : all the safety recommendation, where to throw away what, when to use specific protection and what kind of solutions are dangerous for example. The following rules were applied in the lab:
Biosafety

• Whenever we had to throw cells cultures in the water tank, we first added a special disinfectant (the same used by all the people in the lab) and let it stand for approximately one hour. Only then did we dispose of the cultures. Disinfectant : Environ Vesphene se
• For all other bio contaminated products, we used a special trash bin that was diposed every night by a special facility of the faculty.
• We used gloves for all the expermiments.
• Gloves were not worn outside the lab.

Chemical safety

• Harmful chemicals were kept in special storage place. All toxic and volatile chemicals were handled under a fume hood.
• All material that has been in contact with EtBr (used for agarose gels staining) were diposed of in a special bin. People using that kind of material were wearing nitrile gloves to reinforce there protection.

Our project's safety issues

Our project is considered as a risk Class 1 :

Class 1: GMOs or pathogen agents that are generally speaking harmless for humans.

Indeed there are no specific risks around our project, as we don't work with a pathogenic transgene. ???? to be completed

EPFL Biosafety

Biosafery website

hahaha

iGEM questions

1. Would any of your project ideas raise safety issues in terms of: researcher safety, public safety, or environmental safety?

No

2. Is there a local biosafety group, committee, or review board at your institution?

Yes

3. What does your local biosafety group think about your project?

... -> Aller voir Stéphane Karlen lundi. AAB 025.

4. Do any of the new BioBrick parts that you made this year raise any safety issues?If yes, did you document these issues in the Registry?

No

Conclusions