Team:Groningen/Notebook/21 July 2009

From 2009.igem.org

Wet

GVP Cluster

Transporters

HmtA

The band at ~1150 kb was cut out of the gel and used for pcr described yesterday gave poor results. No band of expected size and strong primerdimer bands Therefor we will redesign the cloning. The Forward primer will be modified into 2 part. which will require an additional PCR but increases the chance of getting product.

Metal Accumulation

Vectors

Positive control for the vector with insert done with both new and last years VR VF2 primers resulting in [http://partsregistry.org/cgi/partsdb/dna_info.cgi?part=BBa%20J61002 1107bp] bands.

This image shows 4 positive results, just the same as yesterdays but slighty higher bands. The above noted PCR was made again only this time without DNA, gel shown below. This indicates that one (VR iGEMGr09) out of 5 primers is not working.

Dry

Jasper finished the GlpF model by fitting a Michaelis-Menten equation to the raw data (using the method of Goudar1999). Mathematica proved to be the best tool for this, as it is one of the few programs that has the Lambert W function built-in (as ProductLog). Furthermore, an interactive model was added to the Wiki demonstrating the results.