Fixing cells with 4% Paraformaldehyde for microscopy on monday
8-31-2009
Microscopy of our first fixed cells transfected with various constructs at the Nikon imaging center
Cells transfected with cmv-GFP, jet-GFP, cmv-jet-GFP
The figure below shows HeLa cells transfected with GFP under a cmv promoter. The image was taken using the Nikon Eclipse 90i upright automated widefield microscope with 40X magnification:
9-2-2009
Microscopy of cells transfected with cmv, jet, cmv-jet with GFP and an mCherry control
Measurement of exposure time versus average grey value to check whether there is a linear correlation between the two. This measurement was performed using a fluorescent slide that has constant fluorescence and doesn't bleach. The correlation factor was calculated to be 0.9998. Therefore we can calculate the grey values of samples with different exposure times.
Evaluation of first microscopy measurements from 8-31-2009 using ImageJ:
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