Team:KU Seoul/Details
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#Inoculation of E. coli DH5a to 5ml LB broth for preparation of competent cell | #Inoculation of E. coli DH5a to 5ml LB broth for preparation of competent cell | ||
#Design of cloning primers & ordering the primers | #Design of cloning primers & ordering the primers | ||
+ | [090915] | ||
+ | #Inoculation of E. coli XL-1 Blue to 2ml LB broth for genomic DNA extraction | ||
+ | #Preparation of E. coli DH5a competent cell (based on BSGC protocol) | ||
+ | [090917] | ||
+ | #Transformation for amplification of BioBrick parts (BBa_E0044, BBa_E1010, pSB3C5 and pSB3T5) (based on BSGC protocol) | ||
+ | #Genomic DNA extraction of E. coli XL-1 Blue by AccuPrep® Genomic DNA Extraction Kit | ||
+ | [090918] | ||
+ | #Inoculation of colonies to 2ml LBAmp(BBa_E0044), LBKan(BBa_E1010), LBCm(pSB3C5) and LBTet(pSB3T5) broth | ||
+ | [090919] | ||
+ | #Plasmid extraction of each part by LaboPass™ Mini Plasmid DNA Purification Kit | ||
+ | #0.8% agarose gel electrophoresis (Figure 1) | ||
+ | |||
+ | [090921] | ||
+ | *PCR of BioBrick Parts | ||
+ | **General PCR condition : 94℃(3’)-[94℃(1’)-53℃(1’)-72℃(3’)]30-72℃(5’)-4℃ | ||
+ | **Results (Figure 2) | ||
+ | **Hot start PCR condition : 95℃(15’)-[95℃(1’)-53℃(1’)-72℃(3’)]30-72℃(10’)-4℃ | ||
+ | **Result (Figure 3) | ||
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Revision as of 05:51, 20 October 2009
The ExperimentsMaterials
Experiment Dairy[090914]
[090915]
[090917]
[090918]
[090919]
[090921]
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