Team:KU Seoul/Details
From 2009.igem.org
(Difference between revisions)
(→Experiment Dairy) |
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*090921 | *090921 | ||
*PCR of BioBrick Parts | *PCR of BioBrick Parts | ||
- | + | *General PCR condition : 94℃(3’)-[94℃(1’)-53℃(1’)-72℃(3’)]30-72℃(5’)-4℃ | |
{| style="color:green; background-color:#ffffcc;" cellpadding="3" cellspacing="0" border="1" | {| style="color:green; background-color:#ffffcc;" cellpadding="3" cellspacing="0" border="1" | ||
! PCR mixture | ! PCR mixture | ||
Line 119: | Line 119: | ||
|D.W. || align="center"| 35.5 | |D.W. || align="center"| 35.5 | ||
|} | |} | ||
- | + | *Hot start PCR condition : 95℃(15’)-[95℃(1’)-53℃(1’)-72℃(3’)]30-72℃(10’)-4℃ | |
- | + | {| style="color:green; background-color:#ffffcc;" cellpadding="3" cellspacing="0" border="1" | |
- | + | ! PCR mixture | |
+ | ! volume (μl) | ||
+ | |- | ||
+ | |Template [mini prep. samples of plasmid : pSB3C5 and pSB3T5] ||align="center"| 1 | ||
+ | |- | ||
+ | |2.5mM dNTP ||align="center"| 4 | ||
+ | |- | ||
+ | |forward-primer (10pmol/μl) ||align="center"| 2 | ||
+ | |- | ||
+ | |reverse-primer (10pmol/μl) || align="center"| 2 | ||
+ | |- | ||
+ | |10x Synergy™ buffer || align="center"| 5 | ||
+ | |- | ||
+ | |S5x Q-solution || align="center"| 10 | ||
+ | |- | ||
+ | |Hot start taq™ (Qiagen) (5U/μl) || align="center"| 0.05 | ||
+ | |- | ||
+ | |D.W. || align="center"| 25.5 | ||
+ | |} | ||
+ | *Result (Figure 2) | ||
[[Image:KU_Seoul_2.jpg]] | [[Image:KU_Seoul_2.jpg]] | ||
|} | |} |
Revision as of 06:56, 20 October 2009
The ExperimentsMaterials
Experiment Dairy
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