Team:KU Seoul/Materials

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**Plasmid pSB3C5 with {{part|BBa_J04450}} : 2009 Kit Plate 1 [5C]
**Plasmid pSB3C5 with {{part|BBa_J04450}} : 2009 Kit Plate 1 [5C]
**Plasmid pSB3T5 with {{part|BBa_J04450}} : 2009 Kit Plate 1 [9C]
**Plasmid pSB3T5 with {{part|BBa_J04450}} : 2009 Kit Plate 1 [9C]
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[[Image:pSB3C5.png][Image:pSN3T5.png|center]]
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[[Image:KU_plasmids.png]]
*Promoters
*Promoters

Latest revision as of 11:13, 21 October 2009






Materials

  • Backbone Plasmids
    • Plasmid pSB3C5 with BBa_J04450 : 2009 Kit Plate 1 [5C]
    • Plasmid pSB3T5 with BBa_J04450 : 2009 Kit Plate 1 [9C]

KU plasmids.png

Template Primer Sequence Length(bp)
Plasmid pSB3C5 pSB3C5_F gctgctgttTAATAAtactagtagcggccgctgc 34
pSB3C5(+Pars)_R taataggtgtgaattttgagttggCtctagaagcggccgcga 42
Plasmid pSB3T5 pSB3T5_F gacgaaaactacgctgctgctgttTAATAAtactagtagcggccgctgc 49
pSB3T5_R GTCGGCAATATGAAGctctagaagcggccgcga 33
Promoter yodA PyodA_F cggccgcttctagagCTTCATATTGCCGACAAAGTACG 38
PyodA_R ATGTGACTTACCCATaacAGTTTCCTCCAGAGTCATTG 38
Green fluorescent protein GR(+Pars)_F aattcacacctattaccttcctctgcacttacacattcgttaagtcatatATGc 78
gtaaaggagaagaacttttcactg
GR(+Pznt)_R ctggagtcgactccagagtcaagttttatcagagatacagcgagcggacg 84
TCTAGTttattaaacagcagcagcgtagttttcg
Red fluorescent protein RF(+Pznt)_F tgataaaacttgactctggagtcgactccagagtgtatccttcggttaatATG 75
gcttcctccgaagacgttatca
RF(+AAV tag)_R TTATTAaacagcagcagcgtagttttcgtcgtttgctgcAgcaccggtgg 59
agtgacgac
Aryl acylamidase AMD_F CTGGAGGAAACTGTTatgGGTAAGTCACATTCGCCAG 37
AMD(+AAV tag)_R TTATTAaacagcagcagcgtagttttcgtcgtttgctgcCAGGGGC 55
CGTCCGGCG
  • Chemicals
    • Acetaminophen (Sigma)
    • CdCl2∙H2O (Junsei)
    • ZnCl2 (Sigma)
    • KH2AsO3 (Sigma)
    • Luria-Bertani broth & Bacto agar (Difco)
    • o-Cresol (Sigma)
    • CuSO4∙5H2O (Sigma)
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