Team:Newcastle/IntroductoryLabwork/30 June 2009

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Introductory Lab Session: 30th June 2009

Our first introductory lab session saw members of the team make preparations to produce some competent DH5-alpha E. coli cells, which will be needed for the numerous transformations that will take place in the near future. The team started by following the first 'Pre-Culture' step in the protocol given to us by Dr. Phil Aldridge and then proceded to make up solutions needed for future steps in this procedure.

Carrying out the first 'Pre-culture' Step

• We got samples of E.coli DH5-alpha strains and plated them into three agar plates. They were placed for incubation at 37°C

Preparing other solutions

• Change to protocol - we have made up Glycerol solution with a concentration of 50% instead of 100%
• We have created 0.1M of CaCl2 using 5.5 gram of CaCl2. The final solution is 0.5 litres. We placed them into 4 bottles (three 100ml and one 200ml). They have blue covers. We have left them for autoclaving.
• We prepared LB solution using 20 grams of LB and water making up a final volume of 1 litre. We put them into bottles and left them for autoclaving.

June M T W T F S S [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/1_June_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/2_June_2009&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/3_June_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/4_June_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/5_June_2009&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/6_June_2009&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/7_June_2009&action=edit 7] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/8_June_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/9_June_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/10_June_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/11_June_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/12_June_2009&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/13_June_2009&action=edit 13] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/14_June_2009&action=edit 14] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/15_June_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/16_June_2009&action=edit 16] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/17_June_2009&action=edit 17] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/18_June_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/19_June_2009&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/20_June_2009&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/21_June_2009&action=edit 21] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/22_June_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/23_June_2009&action=edit 23] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/24_June_2009&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/25_June_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/26_June_2009&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/27_June_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/28_June_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/29_June_2009&action=edit 29] [http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/30_June_2009 30]

July M T W T F S S     [http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/1_July_2009 1] [http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/2_July_2009 2] [http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/3_July_2009 3] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/4_July_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/5_July_2009&action=edit 5] [http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/6_July_2009 6] [http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/7_July_2009 7] [http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/8_July_2009 8] [http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/9_July_2009 9] [http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/10_July_2009 10] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/11_July_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/12_July_2009&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/13_July_2009&action=edit 13] [http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/14_July_2009 14] [http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/15_July_2009 15] [http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/16_July_2009 16] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/17_July_2009&action=edit 17] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/18_July_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/19_July_2009&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/20_July_2009&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/21_July_2009&action=edit 21] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/22_July_2009&action=edit 22] [http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/23_July_2009 23] [http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/24_July_2009 24] [http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/25_July_2009 25] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/26_July_2009&action=edit 26] [http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/27_July_2009 27] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/28_July_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/29_July_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/30_July_2009&action=edit 30] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/31_July_2009&action=edit 31]