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Team:Newcastle/Labwork/11 September 2009
From 2009.igem.org
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** Quantify the midi-prep samples | ** Quantify the midi-prep samples | ||
** Run the midi-prep samples on agarose gel through DNA gel electrophoresis | ** Run the midi-prep samples on agarose gel through DNA gel electrophoresis | ||
| - | * Run products from a PCR reaction involving ''arsR'' and ''cadA'' DNA | + | * '''Run products from a PCR reaction involving ''arsR'' and ''cadA'' DNA''' |
** Run PCR products on gel | ** Run PCR products on gel | ||
** If successful, clean up fragments, run on gel again, cut with restriction enzymes ''NheI'' and ''BamHI'', clean and then ligate together fragments. | ** If successful, clean up fragments, run on gel again, cut with restriction enzymes ''NheI'' and ''BamHI'', clean and then ligate together fragments. | ||
Revision as of 23:49, 13 September 2009
Project
Sub-projects
Wet Lab
Meetings
Planning
Links
Lab Work - 11/09/09
Metal Sensing Team
Introduction and Outline
So far we have transformed DH5-alpha E. coli cells with two of our synthesized BioBricks, which are the cotC-GFP-smtA and the kinA BioBricks. The Metal Sensing team have since taken these transformant colonies and used them to set up LB+kanamycin cultures for mini-preps and midi-preps; these were then left overnight in the orbital shaking incubator and are awaiting processing today. However there are a range of tasks which need to be accomplished today; these are listed below:
- PCR the cotC-GFP-smtA BioBrick with pMK15 and pMK16
- Set up the PCR
- Run the PCR for the correct duration
- Analyse PCR products through DNA gel electrophoresis
- Clean up PCR products if PCR reaction proves to be successful
- Midi-prep cotC-GFP-smtA, kinA, pGFP-rrnB, pMUTIN4 and pSB1AT3
- Carry out the five midi-preps.
- Quantify the midi-prep samples
- Run the midi-prep samples on agarose gel through DNA gel electrophoresis
- Run products from a PCR reaction involving arsR and cadA DNA
- Run PCR products on gel
- If successful, clean up fragments, run on gel again, cut with restriction enzymes NheI and BamHI, clean and then ligate together fragments.
News
Events
- 20 – 21 June 2009 - Europe workshop (London)
- 23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
- 23 October Practice Presentation (Newcastle)
- 23 October T-shirts are ready
- 27 October Practice Presentation (Sunderland)
- 27 October Poster is ready
- 30 October – 2 November 2009 - Jamboree (Boston)
Social Net
