Revision as of 09:46, 9 September 2009

Meetings Calendar

Lab Work - 07/09/09

Metal Sensing Team

Introduction

In last week's lab session, we had received some BBa_J33206 + pSB1A2 DNA in it's original form by Chris French (the person who submitted the BioBrick to the Parts Registry) as well as both PCR and Sequencing primers. Whilst we used the sequencing primers to determine the sequence of the midi-prepped BBa_J33206 DNA derived from the Parts Registry, we also attempted to transform some DH5-alpha E. coli bacteria with the original BBa_J33206 BioBrick sent to us.

In today's lab session, the plates on which the transformed cells were placed will be examined for colonies; if colonies should be present then they will be used to inoculate LB media in preparation for mini-preps.

Observations

Both of the LB+amp plates used to grow the transformant DH5-alpha E. coli cells were covered in hundreds of colonies - almost like a lawn of bacteria could be seen on the plates. This suggests that the transformation rate was very high; this is expected considering we were sent pure DNA. The only thing to note was that the 200ul plate seemed to contain dense, large round colonies in addition to the E. coli cells; this may be contamination.

Procedure

In light of these observations, a total of 6 tubes of 5ml LB solution were innoculated with the transformed E. coli cells. The first three tubes were inoculated by 3 cultures found on the 100ul plate and the next three tubes were inoculated with 3 cultures found on the 200ul plate. This was all done under aspetic conditions.

20 – 21 June 2009 - Europe workshop (London)
23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
23 October Practice Presentation (Newcastle)
23 October T-shirts are ready
27 October Practice Presentation (Sunderland)
27 October Poster is ready
30 October – 2 November 2009 - Jamboree (Boston)

@@ Line 6: / Line 6: @@
 ==<u>Metal Sensing Team</u>==
 ===Introduction===
-In last week's lab session, we had received some ''BBa_J33206'' + ''pSB1A2'' DNA in it's original form by Chris French (the person who submitted the BioBrick to the Parts Registry) as well as both PCR and Sequencing primers. Whilst we used the sequencing primers to determine the sequence of the midi-prepped ''BBa_J33206'' DNA derived from the Parts Registry, we also attempted to tranform some ''DH5-alpha E. coli'' bacteria with the original ''BBa_J33206'' BioBrick sent to us.
+In last week's lab session, we had received some ''BBa_J33206'' + ''pSB1A2'' DNA in it's original form by Chris French (the person who submitted the BioBrick to the Parts Registry) as well as both PCR and Sequencing primers. Whilst we used the sequencing primers to determine the sequence of the midi-prepped ''BBa_J33206'' DNA derived from the Parts Registry, we also attempted to transform some ''DH5-alpha E. coli'' bacteria with the original ''BBa_J33206'' BioBrick sent to us.
 '''In today's lab session, the plates on which the transformed cells were placed will be examined for colonies; if colonies should be present then they will be used to inoculate LB media in preparation for mini-preps.'''
+===Observations===
+Both of the LB+amp plates used to grow the transformant ''DH5-alpha E. coli'' cells were covered in hundreds of colonies - almost like a lawn of bacteria could be seen on the plates. This suggests that the transformation rate was very high; this is expected considering we were sent pure DNA. The only thing to note was that the 200ul plate seemed to contain dense, large round colonies in addition to the ''E. coli'' cells; this may be contamination.
+===Procedure===
+In light of these observations, a total of 6 tubes of 5ml LB solution were innoculated with the transformed ''E. coli'' cells. The first three tubes were inoculated by 3 cultures found on the 100ul plate and the next three tubes were inoculated with 3 cultures found on the 200ul plate. This was all done under aspetic conditions.

Team:Newcastle/Labwork/7 September 2009

From 2009.igem.org