Revision as of 21:14, 19 August 2009

Meetings Calendar

Metal Sensing

==Introduction==] If our project is to process cadmium and not other metals, we need to genetically engineer Bacillus subtilis to carry out a set of cellular processes based on the action of metal sensors. These metal sensors will detect cadmium through a system known as AND Gating.

There are two metal sensing repressors, which are known to respond to cadmium: arsR and czrA. By placing binding sites to these two metal sensing repressors next to each other in a promoter region, the gene regulated by that promoter will be synthesized only when a combination of metals that bind to both sensors are present; this is a combinatorial approach for gene expression regulation.

Plan B allow us to meet a Gold metal criteria: we would improve BBa_J33206 (ArsR) BioBrick.

Modelling

Metal Intake/Efflux Model

@@ Line 4: / Line 4: @@
 __NOTOC__
-=Metal Intake/Efflux=
+=Metal Sensing=
-==Introduction==
+==Introduction==]
+If our project is to process cadmium and not other metals, we need to genetically engineer Bacillus subtilis to carry out a set of cellular processes based on the action of metal sensors. These metal sensors will detect cadmium through a system known as AND Gating.
-For this project we want to be able to process cadmium and cadmium only. Therefore it would be logical to find a way in which we can increase the intake of cadmium without increasing the intake of other metals too.
+There are two metal sensing repressors, which are known to respond to cadmium: arsR and czrA.
+By placing binding sites to these two metal sensing repressors next to each other in a promoter region, the gene regulated by that promoter will be synthesized only when a combination of metals that bind to both sensors are present; this is a combinatorial approach for gene expression regulation.
-It is known that a Bacillus subtilis cell (from the 168 strain) takes up cadmium naturally through the manganese transport system (Laddaga. R.A., Bessen. R., Silver. S.; 1985). Additionally it has been demonstrated that mutations to the manganese transport system can affect its ability to uptake cadmium without affecting its ability to transport manganese (Zeigler. D.R., et al; 1987).
+Plan B allow us to meet a Gold metal criteria: we would improve BBa_J33206 (ArsR) BioBrick.
-The manganese ion channel that we intend to either upregulate or control is the mntH ion channel (also goes under the name ydaR). mntH is part of the Nramp family of proton-coupled, metal ion transporters (Que. Q., Helmann. J.D.; 2000). It is also classified as a secondary transporter (Membrane Transport website).
-It is also known that mntH is regulated negatively by increasing manganese ion concentrations. This is allowed to happen through the promoter called mntR (Que. Q., Helmann. J.D.; 2000). This means that B.subtilis has the ability to limit the manganese metal intake system when the intracellular concentrations of Mn+ starts to near cytotoxic levels.
-The cadA efflux system. In ''B. Subtilis''  cation efflux is by an antiporter. Cd, Co, Zn are pumped out by ATPase dependant systems of the CDF family.
 ==Modelling==
@@ Line 23: / Line 19: @@
 ==BioBrick constructs==
-[[Image:Newcastle Metal Intake Efflux Construct.png|center|500px]]
 ==Lab Work Strategies==

Team:Newcastle/Metalsensing

From 2009.igem.org

Revision as of 21:14, 19 August 2009

Metal Sensing

Modelling

BioBrick constructs

Lab Work Strategies

Other Presentations and Diagrams