Team:PKU Beijing/Notebook/AND Gate 1/Input/AraC

From 2009.igem.org

(Difference between revisions)

Revision as of 20:12, 26 September 2009

Notebook

Notes By Person (PDF)

2009.7.23

10:30
Double-enzyme digestion: 1-23L, 1-2M+AraC

16:50
Gel purification

19:00
Ligation:
Insert: 1-2M+AraC
Vector: 1-23L

23:00
Double-enzyme digestion

2009.7.24

Gel purification

2009.7.27

19:00
Ligation:
Insert: 1-2M+AraC+1-23L
Vector: Pcat

23:00
Transformation

2009.7.28

11:00
PCR: Pcat+1-2M+AraC+1-23L

23:00
Mini-prep and store strains: 1, 2, 4, 5, 6, 7
BioSpec(×50 μg/mL):

1	2.5823
2	2.5939
4	3.0660
5	2.5124
6	2.2350
7	2.2235

00:30
Reverse PCR: Pcat+1-2M+AraC+1-23L

2009.7.29

12:30
Gel purification
BioSpec(×50 μg/mL): 0.1964

14:00
Double-enzyme digestion: Reverse PCR fragment and plasmid of Pcat+1-2M+AraC+1-23L

20:30
Gel purification

22:00
Double-enzyme digestion: 1-1F (Front vector)

2009.7.30

13:30
Gel purification: 1-1F

15:00
Ligation:
Insert: Pcat+1-2M+AraC+1-23L
Vector:1-23L

21:00
Transformation: ligation product

2009.7.31

10:30
PCR

16:00
PCR

23:00
Mini-prep

2009.8.1

12:00
Transformation

2009.8.2

11:30
Pouring plates: LB+Amp ×30, LB+Kan ×50

15:30
Mini-prep: pla1~5, pcr1~4
Gel electrophoresis

17:00
Double-enzyme digestion (front insert)
Plasmid: pla1, pla2, pcr1, pcr3

22:30
Gel purification (pcr1, pla1, pla2)

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+{{PKU_Beijing/Header2}}
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 '''10:30'''<br>
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 Double-enzyme digestion
-=='''2009.7.24'''==
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 Gel purification
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 Transformation
-=='''2009.7.28'''==
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 Mini-prep and store strains: 1, 2, 4, 5, 6, 7<br>
 BioSpec(×50 μg/mL):<br>
-{|style="background:#DDDDDD;" cellpadding=5
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 |1||2.5823
 |-
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 [[Image:PKU_20090728_Rencheng_Gao_2.JPG|400px]]
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 Double-enzyme digestion: 1-1F (Front vector)
-=='''2009.7.30'''==
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 '''13:30'''<br>
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 Transformation: ligation product
-=='''2009.7.31'''==
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 Mini-prep
-=='''2009.8.1'''==
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 Transformation
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 '''11:30'''<br>
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 '''22:30'''<br>
 Gel purification (pcr1, pla1, pla2)<br>
+{{PKU_Beijing/Foot}}
-{{PKU_Beijing/New_End}}
+__NOTOC__