Team:PKU Beijing/Parts

From 2009.igem.org

(Difference between revisions)
(Favourite Parts)
Line 7: Line 7:
</head>
</head>
</html>
</html>
-
==='''Favourite Parts'''===
+
==='''Parts We Contributed This Year'''===
 +
Our team has submitted 7 libraries of well characterized and standardized AND GATE devices. We will contributed several sensor devices and tens of generators or transcriptional units which have function well. More statistical data about the parts we contributed this year are shown in 'Table 1' below.
 +
{|cellpadding=5
 +
|+Table 1. Parameters of the measured transfer functions
 +
| ||Pmax||K||n||R^2
 +
|-
 +
|20 min||29.2±3.6||2.42E-05±1.07E-05||0.710±0.144||0.98124
 +
|-
 +
|40 min||43.4±4.4||6.54E-06±1.48E-06||1.792±0.275||0.97309
 +
|-
 +
|60 min||50.6±6.6||5.13E-06±1.71E-06||1.677±0.335||0.96184
 +
|-
 +
|80 min||58.1±2.5||3.59E-06±1.16E-06||1.520±0.373||0.99088
 +
|-
 +
|100 min||58.1±3.2||3.70E-06±1.05E-06||1.567±0.316||0.98898
 +
|-
 +
|120 min||55.2±0.3||3.32E-06±8.29E-08||1.649±0.032||0.99999
 +
|}
 +
 
==='''Favourite Parts'''===
==='''Favourite Parts'''===

Revision as of 13:46, 21 October 2009

 

Parts We Contributed This Year

Our team has submitted 7 libraries of well characterized and standardized AND GATE devices. We will contributed several sensor devices and tens of generators or transcriptional units which have function well. More statistical data about the parts we contributed this year are shown in 'Table 1' below.

Table 1. Parameters of the measured transfer functions
PmaxKnR^2
20 min29.2±3.62.42E-05±1.07E-050.710±0.1440.98124
40 min43.4±4.46.54E-06±1.48E-061.792±0.2750.97309
60 min50.6±6.65.13E-06±1.71E-061.677±0.3350.96184
80 min58.1±2.53.59E-06±1.16E-061.520±0.3730.99088
100 min58.1±3.23.70E-06±1.05E-061.567±0.3160.98898
120 min55.2±0.33.32E-06±8.29E-081.649±0.0320.99999

Favourite Parts

-?-NameTypeDescriptionDesignerLength
 WBBa_K228000CodingT7ptag(T7polymerase with amber mutation)Lin Min2673
 WBBa_K228001RNASupD-tRNALin Min136
 WBBa_K228004RegulatoryNahR-sal-promoterLin Min1052
 WBBa_K228009RegulatoryAraC protein(reversed sequence) and Pbad promoterGao Rencheng1279
  BBa_K228003DeviceBistable partShan Shen2938


All Parts

-?-NameTypeDescriptionDesignerLength
 WBBa_K228002CompositeK228000-B0015Lin Min2810
 WBBa_K228005GeneratorAraC regulatory protein generatorGao Rencheng1095
 WBBa_K228006GeneratorAraC regulatory protein generatorGao Rencheng1095
 WBBa_K228007GeneratorConstitutive AraC regulatory protein generatorGao Rencheng1141
 WBBa_K228008GeneratorAraC regulatory proteinGao Rencheng1141
 WBBa_K228010ReporterLuxR-LuxP-GFPLin Min1930
  BBa_K228011GeneratorLuxR-LuxP-SupDLin Min1325
 WBBa_K228012GeneratorAraC-Pbad-SupDGao Rencheng1423
 WBBa_K228031GeneratorAraC regulatory protein generator Gao Rencheng1098
  BBa_K228032Translational_UnitRBS and AraC coding sequenceGao Rencheng958
  BBa_K228033Translational_UnitRBS and AraC coding sequenceGao Rencheng958
  BBa_K228034Translational_UnitRBS and AraC coding sequenceGao Rencheng961
  BBa_K228035Translational_UnitRBS and AraC coding sequenceGao Rencheng958
  BBa_K228036Translational_UnitRBS and AraC coding sequenceGao Rencheng958
  BBa_K228037Translational_UnitRBS and AraC coding sequenceGao Rencheng959
  BBa_K228038DeviceAND Gate LuxR-rbs(B0030)-T7ptag-AraC-SupDGao Rencheng5314
  BBa_K228039DeviceAND Gate LuxR-rbs(B0031)-T7ptag-AraC-SupDGao Rencheng5313
   BBa_K228040DeviceAND Gate LuxR-rbs(B0032)-T7ptag-AraC-SupDGao Rencheng5312
   BBa_K228041DeviceAND Gate LuxR-rbs(B0033)-T7ptag-AraC-SupDGao Rencheng5310
  BBa_K228042DeviceAND Gate LuxR-rbs(B0034)-T7ptag-AraC-SupDGao Rencheng5311
  BBa_K228043DeviceAND Gate LuxR-rbs(J44001)-T7ptag-AraC-SupDGao Rencheng5314
  BBa_K228044DeviceAND Gate LuxR-rbs(J61100)-T7ptag-AraC-SupDGao Rencheng5311
  BBa_K228045DeviceAND Gate LuxR-rbs(J61107)-T7ptag-AraC-SupDGao Rencheng5311
  BBa_K228046DeviceAND Gate LuxR-rbs(J61127)-T7ptag-AraC-SupDGao Rencheng5311
   BBa_K228098ReporterT7 promoter+medium RBS+GFPShan Shen930
  BBa_K228099ReporterT7 promoter+strong RBS+GFPShan Shen932
  BBa_K228100CompositeSupD + terminatorShan Shen273
  BBa_K228101GeneratorLacP(R0010) + RBS(B0030) + T7ptag(K228000) + terminator(B0015)Shan Shen3039
   BBa_K228102GeneratorLacP(R0010) + RBS(B0032) + T7ptag(K228000) + terminator(B0015)Shan Shen3037
  BBa_K228103GeneratorLacP(R0010) + RBS(B0034) + T7ptag(K228000) + terminator(B0015)Shan Shen3036
  BBa_K228104GeneratorLacP(R0010) + RBS(J61100) + T7ptag(K228000) + terminator(B0015)Shan Shen3036
  BBa_K228105GeneratorLacP(R0010) + RBS(J61107) + T7ptag(K228000) + terminator(B0015)Shan Shen3036
   BBa_K228106GeneratorLacP(R0010) + RBS(J61127) + T7ptag(K228000) + terminator(B0015)Shan Shen3036
  BBa_K228107GeneratorLacP(R0010) + RBS(J44001) + T7ptag(K228000) + terminator(B0015)Shan Shen3039
   BBa_K228108GeneratorLacP(R0010) + RBS(B0033) + T7ptag(K228000) + terminator(B0015)Shan Shen3035
   BBa_K228109GeneratorLacP(R0010) + RBS(B0031) + T7ptag(K228000) + terminator(B0015)Shan Shen3038
   BBa_K228110GeneratorAND GATE AraC + SupD + LacP +RBS(J61100)+T7ptagShan Shen4604
   BBa_K228111GeneratorAND GATE AraC + SupD + LacP +RBS(J61107)+T7ptagShan Shen4604
   BBa_K228112GeneratorAND GATE AraC + SupD + LacP +RBS(J61127)+T7ptagShan Shen4604
   BBa_K228113GeneratorAND GATE AraC + SupD + LacP +RBS(B0033)+T7ptagShan Shen4603
   BBa_K228114GeneratorAND GATE AraC + SupD + LacP +RBS(JB0031)+T7ptagShan Shen4606
   BBa_K228115GeneratorAND GATE AraC + SupD + LacP +RBS(B0034)+T7ptagShan Shen4604
   BBa_K228116GeneratorAND GATE AraC + SupD + LacP +RBS(B0030)+T7ptagShan Shen4607
   BBa_K228117GeneratorAND GATE AraC + SupD + LacP +RBS(B0032)+T7ptagShan Shen4605
   BBa_K228118GeneratorAND GATE AraC + SupD + LacP +RBS(J44001)+T7ptagShan Shen4607
   BBa_K228119GeneratorAND GATE LacP+RBS(B0030)+T7ptag+LuxP+SupDShan Shen4372
   BBa_K228120GeneratorAND GATE LacP+RBS(B0032)+T7ptag+LuxP+SupDShan Shen4370
   BBa_K228121GeneratorAND GATE LacP+RBS(B0034)+T7ptag+LuxP+SupDShan Shen4369
   BBa_K228122GeneratorAND GATE LacP+RBS(J61100)+T7ptag+LuxP+SupDShan Shen4369
   BBa_K228123GeneratorAND GATE LacP+RBS(J61107)+T7ptag+LuxP+SupDShan Shen4369
   BBa_K228124GeneratorAND GATE LacP+RBS(J611127)+T7ptag+LuxP+SupDShan Shen4369
   BBa_K228125GeneratorAND GATE LacP+RBS(J44001)+T7ptag+LuxP+SupDShan Shen4372
   BBa_K228126GeneratorAND GATE LacP+RBS(B0033)+T7ptag+LuxP+SupDShan Shen4368
   BBa_K228127GeneratorAND GATE LacP+RBS(B0031)+T7ptag+LuxP+SupDShan Shen4371
   BBa_K228200GeneratorRBS->T7ptag->TerminatorGuosheng Zhang2831
   BBa_K228201GeneratorRBS->T7ptag->TerminatorGuosheng Zhang2830
   BBa_K228202GeneratorRBS->T7ptag->TerminatorGuosheng Zhang2829
   BBa_K228203GeneratorRBS->T7ptag->TerminatorGuosheng Zhang2827
   BBa_K228204GeneratorRBS->T7ptag->TerminatorGuosheng Zhang2828
   BBa_K228205GeneratorRBS->T7ptag->TerminatorGuosheng Zhang2831
   BBa_K228206GeneratorRBS->T7ptag->TerminatorGuosheng Zhang2828
   BBa_K228207GeneratorRBS->T7ptag->TerminatorGuosheng Zhang2828
   BBa_K228208GeneratorRBS->T7ptag->TerminatorGuosheng Zhang2828
   BBa_K228209GeneratorAraC->rbs->T7ptag->TerminatorGuosheng Zhang4118
   BBa_K228210GeneratorAraC->rbs->T7ptag->TerminatorGuosheng Zhang4117
   BBa_K228211GeneratorAraC->rbs->T7ptag->TerminatorGuosheng Zhang4116
   BBa_K228212GeneratorAraC->rbs->T7ptag->TerminatorGuosheng Zhang4114
   BBa_K228213GeneratorAraC->rbs->T7ptag->TerminatorGuosheng Zhang4115
   BBa_K228214GeneratorAraC->rbs->T7ptag->TerminatorGuosheng Zhang4118
   BBa_K228215GeneratorAraC->rbs->T7ptag->TerminatorGuosheng Zhang4115
   BBa_K228216GeneratorAraC->rbs->T7ptag->TerminatorGuosheng Zhang4115
   BBa_K228217GeneratorAraC->rbs->T7ptag->TerminatorGuosheng Zhang4115
   BBa_K228218GeneratorLuxR->rbs->T7ptag->TerminatorGuosheng Zhang3883
   BBa_K228219GeneratorLuxR->rbs->T7ptag->TerminatorGuosheng Zhang3882
   BBa_K228220GeneratorLuxR->rbs->T7ptag->TerminatorGuosheng Zhang3881
   BBa_K228221GeneratorLuxR->rbs->T7ptag->TerminatorGuosheng Zhang3879
   BBa_K228222GeneratorLuxR->rbs->T7ptag->TerminatorGuosheng Zhang3880
   BBa_K228223GeneratorLuxR->rbs->T7ptag->TerminatorGuosheng Zhang3883
   BBa_K228224GeneratorLuxR->rbs->T7ptag->TerminatorGuosheng Zhang3880
   BBa_K228225GeneratorLuxR->rbs->T7ptag->TerminatorGuosheng Zhang3880
   BBa_K228226GeneratorLuxR->rbs->T7ptag->TerminatorGuosheng Zhang3880
   BBa_K228227GeneratorSal->rbs->T7ptag->TerminatorGuosheng Zhang3891
   BBa_K228228GeneratorSal->rbs->T7ptag->TerminatorGuosheng Zhang3890
   BBa_K228229GeneratorSal->rbs->T7ptag->TerminatorGuosheng Zhang3889
   BBa_K228230GeneratorSal->rbs->T7ptag->TerminatorGuosheng Zhang3887
   BBa_K228231GeneratorSal->rbs->T7ptag->TerminatorGuosheng Zhang3888
   BBa_K228232GeneratorSal->rbs->T7ptag->TerminatorGuosheng Zhang3891
   BBa_K228233GeneratorSal->rbs->T7ptag->TerminatorGuosheng Zhang3888
   BBa_K228234GeneratorSal->rbs->T7ptag->TerminatorGuosheng Zhang3888
   BBa_K228235GeneratorSal->rbs->T7ptag->TerminatorGuosheng Zhang3888
   BBa_K228236CompositecI->TerminatorGuosheng Zhang912
   BBa_K228237GeneratorRBS->cI->TerminatorGuosheng Zhang933
   BBa_K228238GeneratorRBS->cI->TerminatorGuosheng Zhang932
   BBa_K228239GeneratorRBS->cI->TerminatorGuosheng Zhang931
   BBa_K228240GeneratorRBS->cI->TerminatorGuosheng Zhang929
   BBa_K228241GeneratorRBS->cI->TerminatorGuosheng Zhang930
   BBa_K228242GeneratorRBS->cI->TerminatorGuosheng Zhang933
   BBa_K228243GeneratorRBS->cI->TerminatorGuosheng Zhang930
   BBa_K228244GeneratorRBS->cI->TerminatorGuosheng Zhang930
   BBa_K228245GeneratorRBS->cI->TerminatorGuosheng Zhang930
   BBa_K228246GeneratorT7 promoter->RBS->cI->TerminatorGuosheng Zhang987
   BBa_K228247GeneratorT7 promoter->RBS->cI->TerminatorGuosheng Zhang986
   BBa_K228248GeneratorT7 promoter->RBS->cI->TerminatorGuosheng Zhang985
   BBa_K228249GeneratorT7 promoter->RBS->cI->TerminatorGuosheng Zhang983
   BBa_K228250GeneratorT7 promoter->RBS->cI->TerminatorGuosheng Zhang984
   BBa_K228251GeneratorT7 promoter->RBS->cI->TerminatorGuosheng Zhang987
   BBa_K228252GeneratorT7 promoter->RBS->cI->TerminatorGuosheng Zhang984
   BBa_K228253GeneratorT7 promoter->RBS->cI->TerminatorGuosheng Zhang984
   BBa_K228254GeneratorT7 promoter->RBS->cI->TerminatorGuosheng Zhang984
   BBa_K228255DeviceAND GATE AraC+SupD+Sal+RBS(B0030)+T7ptagGuosheng Zhang5459
   BBa_K228256DeviceAND GATE AraC+SupD+Sal+RBS(B0031)+T7ptagGuosheng Zhang5458
   BBa_K228257DeviceAND GATE AraC+SupD+Sal+RBS(B0032)+T7ptagGuosheng Zhang5457
   BBa_K228258DeviceAND GATE AraC+SupD+Sal+RBS(B0033)+T7ptag Guosheng Zhang5455
   BBa_K228259DeviceAND GATE AraC+SupD+Sal+RBS(B0034)+T7ptagGuosheng Zhang5456
   BBa_K228260DeviceAND GATE AraC+SupD+Sal+RBS(J44001)+T7ptag Guosheng Zhang5459
   BBa_K228261DeviceAND GATE AraC+SupD+Sal+RBS(J61100)+T7ptag Guosheng Zhang5456
   BBa_K228262DeviceAND GATE AraC+SupD+Sal+RBS(J61107)+T7ptag Guosheng Zhang5456
   BBa_K228263DeviceAND GATE AraC+SupD+Sal+RBS(J61127)+T7ptagGuosheng Zhang5456
  BBa_K228801Translational_UnitRBS(B0030)+lacI(C0012)Shuke Wu1174
  BBa_K228802Translational_UnitRBS(B0032)+lacI(C0012)Shuke Wu1172
  BBa_K228803Translational_UnitRBS(B0034)+lacI(C0012)Shuke Wu1171
  BBa_K228804Translational_UnitRBS(J61100)+lacI(C0012)Shuke Wu1171
  BBa_K228805Translational_UnitRBS(J61107)+lacI(C0012)Shuke Wu1171
   BBa_K228806Translational_UnitRBS(J61127)+lacI(C0012)Shuke Wu1171
  BBa_K228807Translational_UnitRBS(B0030)+tetR(C0040)Shuke Wu706
  BBa_K228808Translational_UnitRBS(B0032)+tetR(C0040)Shuke Wu704
  BBa_K228809Translational_UnitRBS(B0034)+tetR(C0040)Shuke Wu703
  BBa_K228810Translational_UnitRBS(J61100)+tetR(C0040)Shuke Wu703
  BBa_K228811Translational_UnitRBS(J61107)+tetR(C0040)Shuke Wu703
   BBa_K228812Translational_UnitRBS(J61127)+tetR(C0040)Shuke Wu703
   BBa_K228813GeneratorRBS(B0034)+lacI(C0012)+terminator(B0015)Shuke Wu1308
   BBa_K228814GeneratorRBS(B0034)+tetR(C0040)+terminator(B0015)Shuke Wu840
  BBa_K228815GeneratorPromoter(constitutive)+RBS(B0034)+lacI(C0012)+terminator(B0015)Shuke Wu1354
  BBa_K228816GeneratorPromoter(constitutive)+RBS(B0034)+tetR(C0040)+terminator(B0015)Shuke Wu886
  BBa_K228817CompositePcat+RBS(B0034)+lacI(C0012)+terminator(B0015)+lacP(R0010)Shuke Wu1562
  BBa_K228818CompositePcat+RBS(B0034)+tetR(C0040)+terminator(B0015)+tetP(R0040)Shuke Wu948
  BBa_K228819CompositePcat+RBS(B0034)+lacI(C0012)+term(B0015)+lacP(R0010)+GFPShuke Wu2448
  BBa_K228820CompositePcat+RBS(B0034)+tetR(C0040)+term(B0015)+tetP(R0040)+GFPShuke Wu1834
  BBa_K228821ReporterlacP(R0010)+GFP(E0840)Shuke Wu1086
   BBa_K228822CompositelacP(R0010)+SupD(K228001)+terminator(B0015) Shuke Wu481
   BBa_K228823DeviceAND GATE lacP+SupD+AraC+RBS(J44001)+T7ptag Shuke Wu4607
  BBa_K228824DeviceAND GATE lacP+SupD+AraC+RBS(B0032)+T7ptag Shuke Wu4605
  BBa_K228825DeviceAND GATE lacP+SupD+AraC+RBS(J61127)+T7ptag Shuke Wu4604
   BBa_K228826DeviceAND GATE lacP+SupD+AraC+RBS(J61107)+T7ptag Shuke Wu4604
  BBa_K228827DeviceAND GATE lacP+SupD+AraC+RBS(B0030)+T7ptag Shuke Wu4607
  BBa_K228828DeviceAND GATE lacP+SupD+AraC+RBS(J61100)+T7ptag Shuke Wu4604
  BBa_K228829DeviceAND GATE lacP+SupD+AraC+RBS(B0033)+T7ptag Shuke Wu4603
  BBa_K228830DeviceAND GATE lacP+SupD+AraC+RBS(B0031)+T7ptag Shuke Wu4606
  BBa_K228831DeviceAND GATE lacP+SupD+AraC+RBS(B0034)+T7ptag Shuke Wu4604
  BBa_K228850GeneratortestHe Siheng273
  BBa_K228851GeneratorSal+supD+terminatorHe Siheng1333
  BBa_K228852DeviceAND GATE Sal+SupD+LacP+RBS(J44001)+T7ptag He Siheng4380
  BBa_K228853DeviceAND GATE Sal+SupD+LacP+RBS(B0030)+T7ptag He Siheng4380
  BBa_K228854DeviceAND GATE Sal+SupD+LacP+RBS(B0032)+T7ptag He Siheng4378
  BBa_K228855DeviceAND GATE Sal+SupD+LacP+RBS(B0034)+T7ptag He Siheng4377
  BBa_K228856DeviceAND GATE Sal+SupD+LacP+RBS(J61100)+T7ptag He Siheng4377
  BBa_K228857DeviceAND GATE Sal+SupD+LacP+RBS(J61107)+T7ptag He Siheng4377
  BBa_K228858DeviceAND GATE Sal+SupD+LacP+RBS(J61127)+T7ptag He Siheng4377
  BBa_K228859DeviceAND GATE Sal+SupD+LacP+RBS(B0033)+T7ptag He Siheng4376
  BBa_K228860DeviceAND GATE Sal+SupD+LacP+RBS(B0031)+T7ptag He Siheng4379
  BBa_K228861GeneratorAraC(K228009) + RBS(B0030) + T7ptag(K228000) + terminator(B0015)Haoqian Zhang4118
  BBa_K228862GeneratorAraC(K228009) + RBS(B0032) + T7ptag(K228000) + terminator(B0015)Haoqian Zhang4116
  BBa_K228863GeneratorAraC(K228009) + RBS(B0034) + T7ptag(K228000) + terminator(B0015)Haoqian Zhang4115
  BBa_K228864GeneratorAraC(K228009) + RBS(J61100) + T7ptag(K228000) + terminator(B0015)Haoqian Zhang4115
  BBa_K228865GeneratorAraC(K228009) + RBS(J61107) + T7ptag(K228000) + terminator(B0015)Haoqian Zhang4115
  BBa_K228866GeneratorAraC(K228009) + RBS(J61127) + T7ptag(K228000) + terminator(B0015)Haoqian Zhang4115
  BBa_K228867GeneratorAraC(K228009) + RBS(J44001) + T7ptag(K228000) + terminator(B0015)Haoqian Zhang4118
  BBa_K228868GeneratorAraC(K228009) + RBS(B0033) + T7ptag(K228000) + terminator(B0015)Haoqian Zhang4114
  BBa_K228869GeneratorAraC(K228009) + RBS(B0031) + T7ptag(K228000) + terminator(B0015)Haoqian Zhang4117
  BBa_K228870GeneratorAND GATE AraC+RBS(B0030)+T7ptag+Sal+SupDHaoqian Zhang5459
  BBa_K228871GeneratorAND GATE AraC+RBS(B0032)+T7ptag+Sal+SupDHaoqian Zhang5457
  BBa_K228872GeneratorAND GATE AraC+RBS(B0034)+T7ptag+Sal+SupDHaoqian Zhang5456
  BBa_K228873GeneratorAND GATE AraC+RBS(J61100)+T7ptag+Sal+SupDHaoqian Zhang5456
  BBa_K228874GeneratorAND GATE AraC+RBS(J61107)+T7ptag+Sal+SupDHaoqian Zhang5456
  BBa_K228875GeneratorAND GATE AraC+RBS(J61127)+T7ptag+Sal+SupDHaoqian Zhang5456
  BBa_K228876GeneratorAND GATE AraC+RBS(J44001)+T7ptag+Sal+SupDHaoqian Zhang5459
  BBa_K228877GeneratorAND GATE AraC+RBS(B0033)+T7ptag+Sal+SupDHaoqian Zhang5455
  BBa_K228878GeneratorAND GATE AraC+RBS(B0031)+T7ptag+Sal+SupDHaoqian Zhang5458



^Top