Team:PKU Beijing/Parts Characterization

From 2009.igem.org

(Difference between revisions)
(Characterization of the AND Gate Part)
(Characterization of the inducible promoters)
Line 9: Line 9:
By assembled with their activators/repressors upstream, inducible promoters may detect the existence of inducers such as arabinose and salicylate. The promoters here act as '''Small Molecule Sensor'''. Two favourite promoter parts involved have been very well characterized.
By assembled with their activators/repressors upstream, inducible promoters may detect the existence of inducers such as arabinose and salicylate. The promoters here act as '''Small Molecule Sensor'''. Two favourite promoter parts involved have been very well characterized.
<br>
<br>
-
(Learn more about the [[Team:PKU_Beijing/Parts_Characterization/BBa_K228004|salicylate promoter]]) and [[Team:PKU_Beijing/Parts_Characterization/BBa_K228009|Pbad promoter]])
+
(Learn more about the [[Team:PKU_Beijing/Parts_Characterization/BBa_K228258|AND GATE AraC+SupD+Sal+RBS(B0033)+T7ptag]] and [[Team:PKU_Beijing/Parts_Characterization/BBa_K228260|Pbad promoter]])
<br><br><br>
<br><br><br>
FOR MORE, PLEASE LOOK AT <br>[[Team:PKU Beijing/Parts Characterization/BBa K228004|'''Salicylate Promoter''']] / [[Team:PKU Beijing/Parts Characterization/BBa K228009|'''Pbad Promoter''']] Pages.
FOR MORE, PLEASE LOOK AT <br>[[Team:PKU Beijing/Parts Characterization/BBa K228004|'''Salicylate Promoter''']] / [[Team:PKU Beijing/Parts Characterization/BBa K228009|'''Pbad Promoter''']] Pages.

Revision as of 16:54, 21 October 2009

 

Characterization of the inducible promoters

PKU GRC AraC 120min.png
PKU BBa K228004 All6 new.png


By assembled with their activators/repressors upstream, inducible promoters may detect the existence of inducers such as arabinose and salicylate. The promoters here act as Small Molecule Sensor. Two favourite promoter parts involved have been very well characterized.
(Learn more about the AND GATE AraC+SupD+Sal+RBS(B0033)+T7ptag and Pbad promoter)


FOR MORE, PLEASE LOOK AT
Salicylate Promoter / Pbad Promoter Pages.

Characterization of the AND Gate Part

PKU AND Gate Mechanism.png



Different promoters are used to drive the expression of T7ptag and SupD tRNA to make an AND Gate. It really needs a great amount of work in order to find one qualified AND Gate out of tens of AND Gates. The figure on the right is the best AND Gate we achieved in the summer. This part is also well characterized. Click here to see.


FOR MORE, PLEASE LOOK AT
AND Gate 1 Design / AND Gate 1 Result Pages



^Top