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Team:TUDelft/17 July 2009
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BBa_K145280 30ml culture induced with 1mM IPTG at 14:40 | BBa_K145280 30ml culture induced with 1mM IPTG at 14:40 | ||
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| + | =='''Calin'''== | ||
| + | Riboregulator scheme 1 and scheme 2 Matlab scripts finished. Ordered lambda red plasmids, (pKD46, pKD3, pKD4) from [http://cgsc2.biology.yale.edu Yale CGSC]. Helped Sriram with transformations. Used DH5alpha comptetent cells to transform:<br> | ||
| + | BBa_I714031 - OriT-R<br> | ||
| + | BBa_J23100 - strong promoter<br> | ||
| + | BBa_E0840 - GFP generator<br> | ||
| + | BBa_I13522 - pTet GFP<br> | ||
| + | |||
{{Template:TUDelftiGEM2009_end}} | {{Template:TUDelftiGEM2009_end}} | ||
Revision as of 15:26, 17 July 2009
17th July
Weenink
9:45 inoculated 30 ml LB amp in 37ºC shaking incubator
pSB1AK3
pSB1AC3
pSB1AT3
11:30 purified plasmids
(in conctrary to protocol i accidentally added >350µl lysis buffer, so i also added 450µl neutralisation buffer) Yield of 100 µl elution:
BBa_B0032: 21.2 ng/µl
BBa_K145015: 33.0 ng/µl
BBa_K145201: 27.8 ng/µl
BBa_K145280 30ml culture induced with 1mM IPTG at 14:40
Calin
Riboregulator scheme 1 and scheme 2 Matlab scripts finished. Ordered lambda red plasmids, (pKD46, pKD3, pKD4) from [http://cgsc2.biology.yale.edu Yale CGSC]. Helped Sriram with transformations. Used DH5alpha comptetent cells to transform:
BBa_I714031 - OriT-R
BBa_J23100 - strong promoter
BBa_E0840 - GFP generator
BBa_I13522 - pTet GFP
