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Team:TUDelft/23 July 2009
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| + | ===Tim Weenink=== | ||
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| + | Details of the following to be provided tomorrow: | ||
| + | |||
| + | grew up *S in LC | ||
| + | |||
| + | looked at gel | ||
| + | |||
| + | Did !A construction with double the amount of backbone (and rest from fridge). Did it with both DH5alpha and homemade top10 chemically competent cells. | ||
| + | |||
| + | PCR'd 8 colonies from my transformation plates with VF2 and I-SceI Reverse primers to see if there was incorporation of the I-SceI restriction site | ||
| + | |||
| + | isolated plasmid from *S | ||
{{Template:TUDelftiGEM2009_end}} | {{Template:TUDelftiGEM2009_end}} | ||
Revision as of 19:04, 23 July 2009
Tim Weenink
Details of the following to be provided tomorrow:
grew up *S in LC
looked at gel
Did !A construction with double the amount of backbone (and rest from fridge). Did it with both DH5alpha and homemade top10 chemically competent cells.
PCR'd 8 colonies from my transformation plates with VF2 and I-SceI Reverse primers to see if there was incorporation of the I-SceI restriction site
isolated plasmid from *S
