"
Team:TUDelft/Lock/Key library
From 2009.igem.org
(Difference between revisions)
Danielsoes (Talk | contribs) (→Lock/Key library) |
Danielsoes (Talk | contribs) (→Lock/Key library) |
||
| Line 7: | Line 7: | ||
As mentioned in preliminary approaches in the section RNA-based Delay: Biosynthetic AND gate, our design was based on the previous work of [https://2009.igem.org/Team:TUDelft/References Isaacs F. 2004], [http://openwetware.org/wiki/IGEM:UC_Berkeley/2006 Berkeley iGEM 2006], [http://openwetware.org/wiki/IGEM:Caltech/2007 Caltech iGEM 2007] and [http://parts.mit.edu/igem07/index.php/Peking Peking iGEM 2007].However, we found that these projects were done using a single RBS. The registry of biobricks has several RBS, besides, as a advice from our modeling team, the RBS present in our constructs should be one with low affinity or “weak” in order to avoid leaky problems. Therefore, we constructed an algorithm based in the previous works which allows us to generate pairs of locks and keys for any RBS (in or outside the registry). | As mentioned in preliminary approaches in the section RNA-based Delay: Biosynthetic AND gate, our design was based on the previous work of [https://2009.igem.org/Team:TUDelft/References Isaacs F. 2004], [http://openwetware.org/wiki/IGEM:UC_Berkeley/2006 Berkeley iGEM 2006], [http://openwetware.org/wiki/IGEM:Caltech/2007 Caltech iGEM 2007] and [http://parts.mit.edu/igem07/index.php/Peking Peking iGEM 2007].However, we found that these projects were done using a single RBS. The registry of biobricks has several RBS, besides, as a advice from our modeling team, the RBS present in our constructs should be one with low affinity or “weak” in order to avoid leaky problems. Therefore, we constructed an algorithm based in the previous works which allows us to generate pairs of locks and keys for any RBS (in or outside the registry). | ||
| - | *[[Team:TUDelft/Lock/Key library: | + | *[[Team:TUDelft/Algorithm | Lock/Key library: Algorithm]] |
| + | |||
| + | *[[Team:TUDelft/New Biobricks | Lock/Key library: New Biobricks Design]] | ||
*[[Team:TUDelft/Results3 | Results]] | *[[Team:TUDelft/Results3 | Results]] | ||
Revision as of 15:17, 16 September 2009
This page is still under construction
Lock/Key library
As mentioned in preliminary approaches in the section RNA-based Delay: Biosynthetic AND gate, our design was based on the previous work of Isaacs F. 2004, Berkeley iGEM 2006, Caltech iGEM 2007 and Peking iGEM 2007.However, we found that these projects were done using a single RBS. The registry of biobricks has several RBS, besides, as a advice from our modeling team, the RBS present in our constructs should be one with low affinity or “weak” in order to avoid leaky problems. Therefore, we constructed an algorithm based in the previous works which allows us to generate pairs of locks and keys for any RBS (in or outside the registry).
