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Team:TUDelft/Lock/Key library
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| - | As mentioned in preliminary approaches in the section RNA-based Delay: Biosynthetic AND gate, our design was based on the previous work of [https://2009.igem.org/Team:TUDelft/ | + | As mentioned in preliminary approaches in the section RNA-based Delay: Biosynthetic AND gate, our design was based on the previous work of [https://2009.igem.org/Team:TUDelft/Module_3_References Isaacs F. 2004], [http://openwetware.org/wiki/IGEM:UC_Berkeley/2006 Berkeley iGEM 2006], [http://openwetware.org/wiki/IGEM:Caltech/2007 Caltech iGEM 2007] and [http://parts.mit.edu/igem07/index.php/Peking Peking iGEM 2007].However, we found that these projects were done using a single RBS. The registry of biobricks has several RBS, besides, as a advice from our modeling team, the RBS present in our constructs should be one with low affinity or “weak” in order to avoid leaky problems. Therefore, we constructed an algorithm based in the previous works which allows us to generate pairs of locks and keys for any RBS (in or outside the registry). |
=='''Lock/Key library: Algorithm'''== | =='''Lock/Key library: Algorithm'''== | ||
Revision as of 21:44, 21 September 2009
Lock/Key library
As mentioned in preliminary approaches in the section RNA-based Delay: Biosynthetic AND gate, our design was based on the previous work of Isaacs F. 2004, Berkeley iGEM 2006, Caltech iGEM 2007 and Peking iGEM 2007.However, we found that these projects were done using a single RBS. The registry of biobricks has several RBS, besides, as a advice from our modeling team, the RBS present in our constructs should be one with low affinity or “weak” in order to avoid leaky problems. Therefore, we constructed an algorithm based in the previous works which allows us to generate pairs of locks and keys for any RBS (in or outside the registry).
Lock/Key library: Algorithm
Based on the various above mentioned studies on the locks and keys, we have developed an algorithm, which had led us to develop a simple web based tool to help in generating the DNA sequences of locks and keys for a chosen Ribosome Binding Site (RBS) DNA sequence. Click here for more information.
Lock/Key generator
You can also directly visit the web based Lock/Key generator page by clicking here.
Lock/Key library: New Biobricks Design
We want to test if the algorithm delivers functional locks and keys. In order to do that, we plan to characterize two locks and keys generated by our algorithm. The RBS’s chosen were the weak and medium (biobricks) from the registry. The DNA sequences of these new parts are... more
