Team:TUDelft/RNA-based Delay, Biosynthetic AND gate: The plan
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='''RNA-based Delay, Biosynthetic AND gate: The plan'''= | ='''RNA-based Delay, Biosynthetic AND gate: The plan'''= | ||
- | Due to the lack of information on siRNA, the enormous advantage and information of riboregulators and the previous work of Isaacs F. 2004, Berkeley iGEM 2006, Caltech iGEM 2006 and Pekin iGEM 2007, the riboregulator approach were selected. The construction of the plasmids is showed in figures 12 and 13. | + | Due to the lack of information on siRNA, the enormous advantage and information of riboregulators and the previous work of [https://2009.igem.org/Team:TUDelft/References Isaacs F. 2004], Berkeley iGEM 2006, Caltech iGEM 2006 and Pekin iGEM 2007, the riboregulator approach were selected. The construction of the plasmids is showed in figures 12 and 13. |
[[Image:Figure12Delay.jpg|thumb|250px|Figure 12. Biosynthetic AND gate’s Plasmid 1. Upon induction with IPTG, the gene cI is transcribed but the Lock3c (biobrick) traps the RBS avoiding the expression of cI. cI will induce the expression of GFP in the plasmid 2.]] | [[Image:Figure12Delay.jpg|thumb|250px|Figure 12. Biosynthetic AND gate’s Plasmid 1. Upon induction with IPTG, the gene cI is transcribed but the Lock3c (biobrick) traps the RBS avoiding the expression of cI. cI will induce the expression of GFP in the plasmid 2.]] |
Revision as of 15:04, 16 September 2009
This page is still under construction
RNA-based Delay, Biosynthetic AND gate: The plan
Due to the lack of information on siRNA, the enormous advantage and information of riboregulators and the previous work of Isaacs F. 2004, Berkeley iGEM 2006, Caltech iGEM 2006 and Pekin iGEM 2007, the riboregulator approach were selected. The construction of the plasmids is showed in figures 12 and 13.