Team:TUDelft/RiboKeyLock Generator

From 2009.igem.org

Revision as of 08:19, 8 August 2009 by Cplesa (Talk | contribs)

Ribo Key & Lock Generator

Enter RBS DNA sequence:
example: TCACACAGGAAACC
or select a BioBrick RBS:




RBS RNA sequence:
example: UCACACAGGAAACC

Key RBS reverse complement RNA sequence with two mismatches:


Lock RBS reverse complement RNA sequence with one mismatch:




Key RNA sequence:

Lock RNA sequence:

Jump to mfold



Key DNA sequence:

Lock DNA sequence:




Key variables:

Upstream

Downstream

Further Downstream (optional part)


Position 1 (bp) of mismatch in the reverse complment of RBS


Position 2 (bp) of mismatch in the reverse complment of RBS




Lock variables:

Upstream

Scar + Start Codon

Mismatch sequence

Scar complement

Position (bp) of mismatch in the reverse complment of RBS