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Team:TUDelft/Synthetic Transcriptional Cascade
From 2009.igem.org
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='''Preliminary approaches'''= | ='''Preliminary approaches'''= | ||
==Negative transcriptional cascade== | ==Negative transcriptional cascade== | ||
| - | [[Team:TUDelft/Preliminary_approaches#Negative_transcriptional_cascade | | + | Based on the work of Hooshangi S et al 2005 [[https://2009.igem.org/Team:TUDelft/References 4]] and the project requirements, the scheme was proposed. |
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| + | Protein 1 starts the circuit activating (or repressing the repressor of) the promoter of gene 2, for proof of concept gene 1 can be changed for a signal molecule. The concentration of protein 2 will increase and achieve the threshold concentration to repress gene 3. At this point... [[Team:TUDelft/Preliminary_approaches#Negative_transcriptional_cascade | more]]. | ||
='''Synthetic Transcriptional Cascade: The plan'''= | ='''Synthetic Transcriptional Cascade: The plan'''= | ||
Revision as of 20:34, 21 September 2009
Transcriptional Cascade
Transcriptional cascades can be seen as temporal programs of successive gene expression [4]. This gene configurations (or circuits) have the capability to integrate signals and trigger events after a delay from the initial detection event. As described before, such characteristic is needed in our project; therefore we considered two approaches based on transcriptional cascades. After some preliminary analysis, the approach termed negative synthetic transcriptional cascade was chosen to be developed.
Preliminary approaches
Negative transcriptional cascade
Based on the work of Hooshangi S et al 2005 [4] and the project requirements, the scheme was proposed.
Protein 1 starts the circuit activating (or repressing the repressor of) the promoter of gene 2, for proof of concept gene 1 can be changed for a signal molecule. The concentration of protein 2 will increase and achieve the threshold concentration to repress gene 3. At this point... more.
Synthetic Transcriptional Cascade: The plan
Synthetic Transcriptional Cascade: The plan
