Team:UNIPV-Pavia/Notebook/Week1Jul

From 2009.igem.org

Revision as of 22:39, 2 July 2009 by Lor18 (Talk | contribs)

EthanolPVanimation.gif

December 2008
M T W T F S S
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31
March 2009
M T W T F S S
1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30 31
April 2009
M T W T F S S
1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
May 2009
M T W T F S S
1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
June 2009
M T W T F S S
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July 2009
M T W T F S S
1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August 2009
M T W T F S S
1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September 2009
M T W T F S S
1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October 2009
M T W T F S S
1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
November 2009
M T W T F S S
1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30

Week from July 1st, to July 5th, 2009

Previous Week Next Week

July, 1st

  • Sequencing results for BOL1 BioBrick: sequence was correct!
  • Miniprep for:
    • A8 (for dilution/transformation)
    • A9 (for dilution/transformation)
  • We transformed 10 pg of A8 and A9 miniprepped DNA in TOP10, after a proper quantification with Nanodrop and dilution. We plated transformed bacteria and incubated them overnight at 37°C.
  • We prepared 0.5 l of LB + Amp.
  • We infected 5 ml of LB + Amp with 10 ul of T9002 glycerol stock. We incubated the culture overnight at 37°C, 220 rpm.
  • We also picked a random colony from A10 plate and infected 1 ml of LB + Amp. We incubated this inoculum at 37°C, 220 rpm for 5 and 1/2 hours. Then we prepared a glycerol stock and re-filled the remaining 250 ul of culture with 5 ml of LB + Amp to grow an overnight culture (for sequencing).


Preparation of experiment with Tecan F200

  • We diluted 1:1000 the cultures of:
    • J23100
    • J23101
    • J23118
    • A1
    • A2
    • A7
    • E0240
  • We incubated the diluted cultures at 37°C, 220 rpm for 3 hours.


Experiment with Tecan F200

  • Description
  • Purpose:
  • Materials & Methods
  • Protocol
  • Results


Top

July, 2nd

  • Miniprep for A10 and T9002. We sent purified DNA to BMR Genomics for sequencing.
  • A8 and A9 (10 pg plates) plates showed colonies!(a few, as expected) We picked 3 colonies for A8 plate and 3 colonies for A9 plate to infect 5 ml of LB + Amp. We incubated the six inocula at 37°C, 220 rpm overnight. Tomorrow they will be miniprepped and screened with digestion to check if the extra band had been lost!



Preparation of experiment with Tecan F200

  • aTc stock preparation.
  • We diluted 1:1000 the cultures of:
    • A8
    • A9 (X2)
    • E0240
  • We incubated the diluted cultures at 37°C, 220 rpm for 3 hours. After 2 hours, one of the two A9 culture was induced with 75 ng/ml aTc and incubated again under the same conditions as before.


Experiment with Tecan F200

  • Description
  • Purpose:
  • Materials & Methods
  • Protocol
  • Results

Top

July, 3rd

  • Miniprep for the overnight cultures (taken from the two 10 pg plates).
  • Digestion E-P for the purified plasmids (under the same conditions as the experiment of June 30th).

Digestion results for A8 and A9 (10 pg plates):

  • Gel results:
  • Tecan F200 data analysis.

Top



Previous Week Next Week