Team:UNIPV-Pavia/Notebook/Week2Aug

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EthanolPVanimation.gif

December 2008
M T W T F S S
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8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31
March 2009
M T W T F S S
1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30 31
April 2009
M T W T F S S
1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
May 2009
M T W T F S S
1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
June 2009
M T W T F S S
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July 2009
M T W T F S S
1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August 2009
M T W T F S S
1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September 2009
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1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October 2009
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5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
November 2009
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30

Week from August 10th, to August 16th, 2009

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August, 10th

  • Digestion for:
B1-13(E-S)(X2) B2-5(E-S)(X2) B3-5(E-X)(X2)
B4-2(E-X)(X2)
  • Gel run for all of them (only 1 ul for B1-13(E-S)(X2) in order to check the length)

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  • Band purification for:
B2-5(E-S)(X2) B3-5(E-X)(X2) B4-2(E-X)(X2)
  • Ethanol precipitation with sodium acetate for B1-13(E-S)(X2).
  • Ligation:
    • B5 = B1(E-S) + B4(E-X) in pSB1AK3
    • B6 = B2(E-S) + B3(E-X) in pSB1AK3
  • We incubated the ligation reactions at 16°C overnight.

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August, 11st

  • Digestion for:
BOL1(E-S) R0010(E-X) F2620MIT1(E-S)
K(E-X)

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August, 12nd

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August, 13rd

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August, 14th

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