Team:UNIPV-Pavia/Notebook/Week2Aug

From 2009.igem.org

Revision as of 10:28, 15 August 2009 by Lor18 (Talk | contribs)

EthanolPVanimation.gif

December 2008
M T W T F S S
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31
March 2009
M T W T F S S
1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30 31
April 2009
M T W T F S S
1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
May 2009
M T W T F S S
1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
June 2009
M T W T F S S
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July 2009
M T W T F S S
1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August 2009
M T W T F S S
1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September 2009
M T W T F S S
1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October 2009
M T W T F S S
1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
November 2009
M T W T F S S
1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30

Week from August 10th, to August 16th, 2009

Previous Week Next Week

August, 10th

  • Digestion for:
B1-13(E-S)(X2) B2-5(E-S)(X2) B3-5(E-X)(X2)
B4-2(E-X)(X2)
  • Gel run for all of them (only 1 ul for B1-13(E-S)(X2) in order to check the length)

Insert here

  • Band purification for:
B2-5(E-S)(X2) B3-5(E-X)(X2) B4-2(E-X)(X2)
  • Ethanol precipitation with sodium acetate for B1-13(E-S)(X2).
  • Ligation:
    • B5 = B1(E-S) + B4(E-X) in pSB1AK3
    • B6 = B2(E-S) + B3(E-X) in pSB1AK3
  • We incubated the ligation reactions at 16°C overnight.

Top

August, 11st

  • We transformed the overnight ligations of B5 and B6. We plated the transformed bacteria on LB agar plates + Kan and incubated the plates at 37°C overnight.


  • Digestion for:
BOL1(E-S) R0010(E-X) F2620MIT1(E-S)
K112808(E-X)
  • Gel run/cut/purification for all of them. All the bands were present at the right size!
  • Ligation (20 ul final volume) for:
    • A11 = BOL1(E-S) + R0011(E-X) in pSB1A2 (we call it A11 again, because the previous A11 had a deletion ad we threw it away)
    • A15 = F2620MIT1(E-S) + K112808(E-X) in pSB1A2
  • We incubated the ligations at 16°C overnight.


  • M9 supplemented medium preparation (with glycerol). This first attempt showed precipitations...we will try tomorrow to dissolve MgSO4 and CaCL2 in ddH2O very slowly.

Top

August, 12nd

Top

August, 13rd

Top

August, 14th

Top


Previous Week Next Week