Team:UNIPV-Pavia/Notebook/Week2Jul

From 2009.igem.org

Revision as of 11:28, 7 July 2009 by Lor18 (Talk | contribs)

EthanolPVanimation.gif

December 2008
M T W T F S S
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31
March 2009
M T W T F S S
1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30 31
April 2009
M T W T F S S
1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
May 2009
M T W T F S S
1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
June 2009
M T W T F S S
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July 2009
M T W T F S S
1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August 2009
M T W T F S S
1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September 2009
M T W T F S S
1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October 2009
M T W T F S S
1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
November 2009
M T W T F S S
1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30

Week from July 6th, to July 12nd, 2009

Previous Week Next Week

July, 6th

  • Digestion for:
R0011(E-X) BOL1(E-S)
  • Gel run/cut and band purification.
  • The purified DNA gave a good result at Nanodrop, but we decided to perform the entire work on BOL1 again because we were not sure that we had extracted a pure band.
  • We stored R0011(E-X) DNA at -20°C.
  • We infected 5 ml of LB + Amp with 8 ul of BOL1 glycerol stock to grow an overnight culture (37°C, 220 rpm).

Preparation of experiment with Tecan F200

  • We picked a colony of B0030 plate (stored at +4°C) and infected 5 ml of LB + Amp.
  • We also infected 5 ml of LB + Amp with 10 ul of A1 and J23100 glycerol stocks.
  • We incubated the inocula overnight at 37°C, 220 rpm.

Top

July, 7th

  • Miniprep for BOL1 (X2).
  • Digestion for BOL1(E-S)(X2).
  • Gel run/cut/purification.
  • Ligation:
    • A11 = BOL1(E-S) + R0011(E-X) in pSB1A2
  • We incubated the ligation overnight at 16°C.


Preparation of experiment with Tecan F200

  • We diluted 1:100 the overnight cultures of A1, B0030 and J23100.
  • We incubated the diluted cultures for 5 hours (37°C, 220 rpm).

Experiment with Tecan F200

Top

July, 8th

Top

July, 9th

Top

July, 10th

Top



Previous Week Next Week