Team:UNIPV-Pavia/Notebook/Week2Sep

From 2009.igem.org

Revision as of 16:57, 28 September 2009 by Elisa df (Talk | contribs)

EthanolPVanimation.gif

December 2008
M T W T F S S
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31
March 2009
M T W T F S S
1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30 31
April 2009
M T W T F S S
1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
May 2009
M T W T F S S
1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
June 2009
M T W T F S S
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30
July 2009
M T W T F S S
1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30 31
August 2009
M T W T F S S
1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31
September 2009
M T W T F S S
1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
October 2009
M T W T F S S
1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
November 2009
M T W T F S S
1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30

Week from Semptember 7th, to September 13th, 2009

Previous Week Next Week

September, 7th

In the morning we have prepared colture of A2 to stock in glycerol and we have prepared LB medium.

In the afternoon we have incubated in adequate medium:


  • B9 (10 ml LB+Amp)
  • B10 (10 ml LB+Amp)
  • T9002 (10 ml LB + Amp)

for a qualitative test of ethanol production,


  • A9pg (5 ml LB + Amp)
  • A16 (5 ml LB + Amp)
  • A8 (5 ml LB + Amp)
  • A2 (5 ml LB + Amp)
  • RBS33 (5 ml LB + Amp)

to perform an aTc induction test,


  • A15 (25 ml LB + Amp)
  • pSB3K3 (25 ml LB + Kan)
  • A14-1 L (5 ml LB + Amp)

to miniprep tomorrow.


These coltures have been incubated at 37°C, 220 rpm overnight. Tomorrow, we will miniprep A14-1 L and midiprep A15 and 3K3 (pSB3K3). We perform midiprep on pSB3K3 because it is a low copy number plasmid and on A15 because it is a lysis cassette, so for leackage only "few" cells survive.

Glycerol stocks of A2-3 have been stored at -80°C, named A2-3 and A2-3 bis.The previous stock has been thrown away.

Preparation of a qualitative test to assay the presence of ethanol

We have also induced coltures B9 and B10 incubated yesterday morning at 37°C 220 rpm in flasks and grown overnight. In the morning the coltures have been diluited to reach the desired OD (0.03), then re-incubated for 4 hours and induced with 3OC6-HSL 10nM. The induced coltures have been incubated at 18.00 o'clock and will stay overnight. Flasks have been ermetically closed, to allow fermentation. This qualitative test is performed to assay the presence oh ethanol.

Tomorrow we will use the "Ethanol assay kit" to test the presence of ethanol!!

Top

September, 8th

Top

September, 9th

Top

September, 10th

Top

September, 11st

At 16.00, meeting of our team with representatives of Costa Foundation.

Top

September, 12nd

Top

September, 13rd

Top


Previous Week Next Week