Team:UNIPV-Pavia/Notebook/Week3Sep

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== <html><font class="dayw_style">September, 18th</font></html> ==
== <html><font class="dayw_style">September, 18th</font></html> ==
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===== pH sensor =====
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*We put 50 ul into 5 ml LBK + Amp falcon for each of the overnight cultures RBS33 - A2 - pNhaA and incubated them for four hours and a half.
 +
*OD measure of the three falcon with TECAN in order to make dilutions with a final volume of 3 ml and an OD equal to 0,02.
 +
*We diluted:
 +
**RBS into:
 +
*** LBK + Amp pH 5,5
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*** LBK + Amp pH 6,6
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*** LBK + Amp pH 7,5
 +
*** LBK + Amp pH 8,5
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**pNhaA into:
 +
*** LBK + Amp pH 5,5
 +
*** LBK + Amp pH 6,6
 +
*** LBK + Amp pH 7,5
 +
*** LBK + Amp pH 8,5
 +
**A2 into:
 +
*** LBK + Amp pH 5,5
 +
*** LBK + Amp pH 6,6
 +
*** LBK + Amp pH 7,5
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*** LBK + Amp pH 8,5

Revision as of 10:07, 11 October 2009

EthanolPVanimation.gif

December 2008
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March 2009
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April 2009
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May 2009
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June 2009
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29 30
July 2009
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27 28 29 30 31
August 2009
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24 25 26 27 28 29 30
31
September 2009
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7 8 9 10 11 12 13
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21 22 23 24 25 26 27
28 29 30
October 2009
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12 13 14 15 16 17 18
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November 2009
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30

Week from September 14th, to September 20th, 2009

Previous Week Next Week

September, 14th

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September, 15th

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September, 16th

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September, 17th

pH sensor
  • Infection of 4 ml LB + Amp with 8 ul from glycerol stock of:
    • RBS33
    • A2
    • pNhaA + GFP (from now on it we'll be indicated only as pNhaA)
  • Overnight incubation of the three culctures.


Top

September, 18th

pH sensor
  • We put 50 ul into 5 ml LBK + Amp falcon for each of the overnight cultures RBS33 - A2 - pNhaA and incubated them for four hours and a half.
  • OD measure of the three falcon with TECAN in order to make dilutions with a final volume of 3 ml and an OD equal to 0,02.
  • We diluted:
    • RBS into:
      • LBK + Amp pH 5,5
      • LBK + Amp pH 6,6
      • LBK + Amp pH 7,5
      • LBK + Amp pH 8,5
    • pNhaA into:
      • LBK + Amp pH 5,5
      • LBK + Amp pH 6,6
      • LBK + Amp pH 7,5
      • LBK + Amp pH 8,5
    • A2 into:
      • LBK + Amp pH 5,5
      • LBK + Amp pH 6,6
      • LBK + Amp pH 7,5
      • LBK + Amp pH 8,5


Top

September, 19th

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September, 20th

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