Team:UNIPV-Pavia/Notebook/Week4Jul
From 2009.igem.org
(Difference between revisions)
(→July, 20th) |
(→July, 20th) |
||
Line 24: | Line 24: | ||
== <html><font class="dayw_style">July, 20th</font></html> == | == <html><font class="dayw_style">July, 20th</font></html> == | ||
+ | |||
+ | *Overnight digestion (20 ul reaction volume) for: | ||
+ | {|cellpadding="20" | ||
+ | |MRGENE1(X-P) | ||
+ | |MRGENE1-2(X-P) | ||
+ | |MRGENE1-3(X-P) | ||
+ | |- | ||
+ | |MRGENE2(X-P)(X2) | ||
+ | |MRGENE2-2(X-P) | ||
+ | |MRGENE2-3(X-P) | ||
+ | |- | ||
+ | |B0030(S-P)(X3) | ||
+ | |} | ||
+ | *NOTE: reactions which involve excision(X-P) were performed on 3 ug of DNA, while S-P digestions were performed with 1 ug of DNA. We decided to have 3 replicates to be sure to have an acceptable yield. | ||
+ | |||
+ | *We decided to perform gel run/cut/purification for MRGENE2 and for B0030, while we decided to perform DNA precipitation with sodium acetate for MRGENE1. | ||
+ | |||
<div align="right"> | <div align="right"> |
Revision as of 20:03, 20 July 2009
|
|
|
|
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|
|
|
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|
Week from July 20th, to July 26th, 2009
Previous Week | Next Week |
July, 20th
- Overnight digestion (20 ul reaction volume) for:
MRGENE1(X-P) | MRGENE1-2(X-P) | MRGENE1-3(X-P) |
MRGENE2(X-P)(X2) | MRGENE2-2(X-P) | MRGENE2-3(X-P) |
B0030(S-P)(X3) |
- NOTE: reactions which involve excision(X-P) were performed on 3 ug of DNA, while S-P digestions were performed with 1 ug of DNA. We decided to have 3 replicates to be sure to have an acceptable yield.
- We decided to perform gel run/cut/purification for MRGENE2 and for B0030, while we decided to perform DNA precipitation with sodium acetate for MRGENE1.
July, 21st
July, 22nd
July, 23rd
July, 24th
Previous Week | Next Week |