Team:UNIPV-Pavia/Notebook/Week5Jul
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- | *NOTE: we had a pure sample for B1 and a almost-pure sample for B2. Anyway, we decided to perform ligation reactions for these samples and the extra band of B2 will be eliminated during gel cut/purification. Next weeks we will think about purifying B2 itself. | + | *NOTE: we had a pure sample for B1 and a almost-pure sample for B2. Anyway, we decided to perform ligation reactions for these samples and the extra band of B2 will be eliminated during gel cut/purification. WE DECIDED TO KEEP B2-5. Next weeks we will think about purifying B2-5 itself. |
Revision as of 12:44, 4 August 2009
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Week from July 27th, to August 1st, 2009
Previous Week | Next Week |
July, 27th
- Screening for the 11 miniprepped DNA samples for B1: digestion S-P for all.
- Gel results:
- Samples 1, 3, 5, 6, 8, 10 and 11 showed an extra band for the non ligated plasmid;
- Samples 2, 4, 7 and 9 were pure! we decided to keep B1-7 to perform future ligations.
- NOTE: we had a pure sample for B1 and a almost-pure sample for B2. Anyway, we decided to perform ligation reactions for these samples and the extra band of B2 will be eliminated during gel cut/purification. WE DECIDED TO KEEP B2-5. Next weeks we will think about purifying B2-5 itself.
Preparation of experiment with Tecan F200
- We infected 5 ml of LB + Amp with 10 ul of A14pg, B0030, A8pg and A9pg glycerol stocks.
- We incubated the inoculum at 37°C, 220 rpm overnight.
July, 28th
July, 29th
July, 30th
July, 31st
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