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Team:UNIPV-Pavia/Notebook/Week5Sep
From 2009.igem.org
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== <html><font class="dayw_style">September, 29th</font></html> == | == <html><font class="dayw_style">September, 29th</font></html> == | ||
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| + | *A14 induction test using IPTG | ||
| + | The part doens't show any induction!! | ||
| + | To test what happened, we have inoculated A14 L-1 from glycerol stocks. tomorrow it will be diluted 1:100 in 2 falcons (5ml), one of wich will be induced 10nM. | ||
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| + | *Inoculus of T9002 from glycerol stocks in 2 falons, one grown anaerobically and the other areobically. They have been induced with 3OC6HSL 10nM and the next morning the measure of GFP was about the half in the anaerobiotically grown culture respect to the aerobically grown one. | ||
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| + | *Prepartion of 2 falcons 50ml containing 35ml LB+Amp+2%glucose and infected from glycerol stocks with B9 and F2620. They have been incubated overnight at 37°C statically, without shaking. | ||
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Revision as of 14:10, 2 October 2009
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Week from September 28th, to October 4th, 2009
Previous Week
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Next Week
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September, 28th
September, 29th
- A14 induction test using IPTG
The part doens't show any induction!! To test what happened, we have inoculated A14 L-1 from glycerol stocks. tomorrow it will be diluted 1:100 in 2 falcons (5ml), one of wich will be induced 10nM.
- Inoculus of T9002 from glycerol stocks in 2 falons, one grown anaerobically and the other areobically. They have been induced with 3OC6HSL 10nM and the next morning the measure of GFP was about the half in the anaerobiotically grown culture respect to the aerobically grown one.
- Prepartion of 2 falcons 50ml containing 35ml LB+Amp+2%glucose and infected from glycerol stocks with B9 and F2620. They have been incubated overnight at 37°C statically, without shaking.
September, 30th
October, 1st
October, 2nd
October, 3rd
October, 4th
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Next Week
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