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Team:UQ-Australia/Notebook
From 2009.igem.org
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'''6/08/09''' | '''6/08/09''' | ||
| - | Plasmid solutions were left overnight and in the morning checked again with Nanodrop. No DNA was measured. Samples were put in the waterbath at 37 degrees and left for a couple of hours. | + | |
| + | Plasmid solutions were left overnight and in the morning checked again with Nanodrop. No DNA was measured. Samples were put in the waterbath at 37 degrees and left for a couple of hours. If Nanodrop does not work after the waterbath we will still do transformation since we only need few plasmids for the transformation to work. Nanodrop may not have been able to quantify such a small concentration of plasmids. | ||
Revision as of 23:33, 5 August 2009
Water Purification ProjectDATE What was done |
Bioprecipitation Project5/08/09 Plasmids have arrived! We have put them into 150 mL of TE buffer and left overnight for the paper to dissolve and later extract the plasmid from the solution. A Nanodrop was used to see if the DNA was coming out of the paper. No DNA was detected, thus samples were left overnight. Click on the plasmid name to look at the vector
6/08/09 Plasmid solutions were left overnight and in the morning checked again with Nanodrop. No DNA was measured. Samples were put in the waterbath at 37 degrees and left for a couple of hours. If Nanodrop does not work after the waterbath we will still do transformation since we only need few plasmids for the transformation to work. Nanodrop may not have been able to quantify such a small concentration of plasmids. |
