Team:Uppsala-Sweden/Notebook

From 2009.igem.org

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==Oligonucleotides used by the Uppsala iGEM Team 2009==
 
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[[Media:iGEMUppsala09_Primer.xls]]
 
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== '''Notebook''' ==
 
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==Notebook==
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==Oligonucleotides used by the Uppsala iGEM Team 2009==
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All primers we used during the project can be found here: [[Media:iGEMUppsala09_Primer.xls|iGEMUppsala09_Primer.xls]]<br>
 +
Annealing temperatures etc. can be found in the notebook.
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==Protocols==
 +
 +
====PCR Setup====
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[[Media:PCR MM and Primers.xls|PCR MM and Primers.xls]]
 +
 +
====Transformation Protocol====
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[[Media:Transformation workorder.pdf|Transformation workorder.pdf]]
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 +
====Growth media for <i>Z. mobilis</i>====
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[[Media:Fullmedium mobilis.xls|Fullmedium mobilis.xls]]<br>
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[[Media:Fullmedium mobilis plus Agar.xls|Fullmedium mobilis plus Agar.xls]]
 +
 +
====Growth medium for <i>Synechocystis sp PCC 6803</i>====
 +
[[Media:BG11.pdf|BG11.pdf]]
 +
 +
====Growth medium for <i>E. coli</i>====
 +
[[Media:LB.pdf|LB.pdf]]
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 +
====Various====
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[[Media:Glycerol Stocks.xls|Glycerol Stocks.xls]]<br>
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[[Media:Triparental_Mating.pdf|Triparental_Mating.pdf]]
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__NOTOC__
{{Uppsala-Sweden_Footer}}
{{Uppsala-Sweden_Footer}}

Latest revision as of 02:42, 22 October 2009




Notebook

June
MTWTFSS
[http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/1_June_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/2_June_2009&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/3_June_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/4_June_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/5_June_2009&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/6_June_2009&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/7_June_2009&action=edit 7]
[http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/8_June_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/9_June_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/10_June_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/11_June_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/12_June_2009&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/13_June_2009&action=edit 13] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/14_June_2009&action=edit 14]
[http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/15_June_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/16_June_2009&action=edit 16] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/17_June_2009&action=edit 17] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/18_June_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/19_June_2009&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/20_June_2009&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/21_June_2009&action=edit 21]
[http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/22_June_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/23_June_2009&action=edit 23] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/24_June_2009&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/25_June_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/26_June_2009&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/27_June_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/28_June_2009&action=edit 28]
[http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/29_June_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/30_June_2009&action=edit 30]
July
MTWTFSS
    [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/1_July_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/2_July_2009&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/3_July_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/4_July_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/5_July_2009&action=edit 5]
[http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/6_July_2009&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/7_July_2009&action=edit 7] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/8_July_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/9_July_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/10_July_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/11_July_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/12_July_2009&action=edit 12]
[http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/13_July_2009&action=edit 13] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/14_July_2009&action=edit 14] [http://2009.igem.org/Uppsala-Sweden/15_July_2009 15] [http://2009.igem.org/Uppsala-Sweden/16_July_2009 16] [http://2009.igem.org/Uppsala-Sweden/17_July_2009 17] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/18_July_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/19_July_2009&action=edit 19]
[http://2009.igem.org/Uppsala-Sweden/20_July_2009 20] [http://2009.igem.org/Uppsala-Sweden/21_July_2009 21] [http://2009.igem.org/Uppsala-Sweden/22_July_2009 22] [http://2009.igem.org/Uppsala-Sweden/23_July_2009 23] [http://2009.igem.org/Uppsala-Sweden/24_July_2009 24] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/25_July_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/26_July_2009&action=edit 26]
[http://2009.igem.org/Uppsala-Sweden/27_July_2009 27] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/28_July_2009&action=edit 28] [http://2009.igem.org/Uppsala-Sweden/29_July_2009 29] [http://2009.igem.org/Uppsala-Sweden/30_July_2009 30] [http://2009.igem.org/Uppsala-Sweden/31_July_2009 31]
August
MTWTFSS
          [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/1_August_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/2_August_2009&action=edit 2]
[http://2009.igem.org/Uppsala-Sweden/3_August_2009 3] [http://2009.igem.org/Uppsala-Sweden/4_August_2009 4] [http://2009.igem.org/Uppsala-Sweden/5_August_2009 5] [http://2009.igem.org/Uppsala-Sweden/6_August_2009 6] [http://2009.igem.org/Uppsala-Sweden/7_August_2009 7] [http://2009.igem.org/Uppsala-Sweden/8_August_2009 8] [http://2009.igem.org/Uppsala-Sweden/9_August_2009 9]
[http://2009.igem.org/Uppsala-Sweden/10_August_2009 10] [http://2009.igem.org/Uppsala-Sweden/11_August_2009 11] [http://2009.igem.org/Uppsala-Sweden/12_August_2009 12] [http://2009.igem.org/Uppsala-Sweden/13_August_2009 13] [http://2009.igem.org/Uppsala-Sweden/14_August_2009 14] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/15_August_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/16_August_2009&action=edit 16]
[http://2009.igem.org/Uppsala-Sweden/17_August_2009 17] [http://2009.igem.org/Uppsala-Sweden/18_August_2009 18] [http://2009.igem.org/Uppsala-Sweden/19_August_2009 19] [http://2009.igem.org/Uppsala-Sweden/20_August_2009 20] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/21_August_2009&action=edit 21] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/22_August_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/23_August_2009&action=edit 23]
[http://2009.igem.org/Uppsala-Sweden/24_August_2009 24] [http://2009.igem.org/Uppsala-Sweden/25_August_2009 25] [http://2009.igem.org/Uppsala-Sweden/26_August_2009 26] [http://2009.igem.org/Uppsala-Sweden/27_August_2009 27] [http://2009.igem.org/Uppsala-Sweden/28_August_2009 28] [http://2009.igem.org/Uppsala-Sweden/29_August_2009 29] [http://2009.igem.org/Uppsala-Sweden/30_August_2009 30]
[http://2009.igem.org/Uppsala-Sweden/31_August_2009 31]
September
MTWTFSS
  [http://2009.igem.org/Uppsala-Sweden/1_September_2009 1] [http://2009.igem.org/Uppsala-Sweden/2_September_2009 2] [http://2009.igem.org/Uppsala-Sweden/3_September_2009 3] [http://2009.igem.org/Uppsala-Sweden/4_September_2009 4] [http://2009.igem.org/Uppsala-Sweden/5_September_2009 5] [http://2009.igem.org/Uppsala-Sweden/6_September_2009 6]
[http://2009.igem.org/Uppsala-Sweden/7_September_2009 7] [http://2009.igem.org/Uppsala-Sweden/8_September_2009 8] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/9_September_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/10_September_2009&action=edit 10] [http://2009.igem.org/Uppsala-Sweden/11_September_2009 11] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/12_September_2009&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/13_September_2009&action=edit 13]
[http://2009.igem.org/Uppsala-Sweden/14_September_2009 14] [http://2009.igem.org/Uppsala-Sweden/15_September_2009 15] [http://2009.igem.org/Uppsala-Sweden/16_September_2009 16] [http://2009.igem.org/Uppsala-Sweden/17_September_2009 17] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/18_September_2009&action=edit 18] [http://2009.igem.org/Uppsala-Sweden/19_September_2009 19] [http://2009.igem.org/Uppsala-Sweden/20_September_2009 20]
[http://2009.igem.org/Uppsala-Sweden/21_September_2009 21] [http://2009.igem.org/Uppsala-Sweden/22_September_2009 22] [http://2009.igem.org/Uppsala-Sweden/23_September_2009 23] [http://2009.igem.org/Uppsala-Sweden/24_September_2009 24] [http://2009.igem.org/Uppsala-Sweden/25_September_2009 25] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/26_September_2009&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/27_September_2009&action=edit 27]
[http://2009.igem.org/Uppsala-Sweden/28_September_2009 28] [http://2009.igem.org/Uppsala-Sweden/29_September_2009 29] [http://2009.igem.org/Uppsala-Sweden/30_September_2009 30]
October
MTWTFSS
      [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/1_October_2009&action=edit 1] [http://2009.igem.org/Uppsala-Sweden/2_October_2009 2] [http://2009.igem.org/Uppsala-Sweden/3_October_2009 3] [http://2009.igem.org/Uppsala-Sweden/4_October_2009 4]
[http://2009.igem.org/Uppsala-Sweden/5_October_2009 5] [http://2009.igem.org/Uppsala-Sweden/6_October_2009 6] [http://2009.igem.org/Uppsala-Sweden/7_October_2009 7] [http://2009.igem.org/Uppsala-Sweden/8_October_2009 8] [http://2009.igem.org/Uppsala-Sweden/9_October_2009 9] [http://2009.igem.org/Uppsala-Sweden/10_October_2009 10] [http://2009.igem.org/Uppsala-Sweden/11_October_2009 11]
[http://2009.igem.org/Uppsala-Sweden/12_October_2009 12] [http://2009.igem.org/Uppsala-Sweden/13_October_2009 13] [http://2009.igem.org/Uppsala-Sweden/14_October_2009 14] [http://2009.igem.org/Uppsala-Sweden/15_October_2009 15] [http://2009.igem.org/Uppsala-Sweden/16_October_2009 16] [http://2009.igem.org/Uppsala-Sweden/17_October_2009 17] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/18_October_2009&action=edit 18]
[http://2009.igem.org/Uppsala-Sweden/19_October_2009 19] [http://2009.igem.org/Uppsala-Sweden/20_October_2009 20] [http://2009.igem.org/Uppsala-Sweden/21_October_2009 21] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/22_October_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/23_October_2009&action=edit 23] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/24_October_2009&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/25_October_2009&action=edit 25]
[http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/26_October_2009&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/27_October_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/28_October_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/29_October_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/30_October_2009&action=edit 30] [http://2009.igem.org/wiki/index.php?title=Uppsala-Sweden/31_October_2009&action=edit 31]


Oligonucleotides used by the Uppsala iGEM Team 2009

All primers we used during the project can be found here: iGEMUppsala09_Primer.xls
Annealing temperatures etc. can be found in the notebook.

Protocols

PCR Setup

PCR MM and Primers.xls

Transformation Protocol

Transformation workorder.pdf

Growth media for Z. mobilis

Fullmedium mobilis.xls
Fullmedium mobilis plus Agar.xls

Growth medium for Synechocystis sp PCC 6803

BG11.pdf

Growth medium for E. coli

LB.pdf

Various

Glycerol Stocks.xls
Triparental_Mating.pdf






Bioneer Biolegio Clontech Uppsala Genome Center