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Team:Warsaw/Primers
From 2009.igem.org
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!name | !name | ||
!sequence | !sequence | ||
| - | !style="text-align: | + | !style="text-align: left;"|description |
|- | |- | ||
|width="10%"|<html><a name="croboxF">croboxF</a></html> | |width="10%"|<html><a name="croboxF">croboxF</a></html> | ||
|ATCTAGATACCTCTGGCGGTGATACTAGTGT | |ATCTAGATACCTCTGGCGGTGATACTAGTGT | ||
| - | |style="text-align: | + | |style="text-align: left;"|forward primer for [https://2009.igem.org/Team:Warsaw/Glossary#cro-box cro-box] |
|- | |- | ||
|<html><a name="croboxR">croboxR</a></html> | |<html><a name="croboxR">croboxR</a></html> | ||
|ACACTAGTATCACCGCCAGAGGTATCTAGAT | |ACACTAGTATCACCGCCAGAGGTATCTAGAT | ||
| - | |style="text-align: | + | |style="text-align: left;"|reverse primer for [https://2009.igem.org/Team:Warsaw/Glossary#cro-box cro-box] |
|- | |- | ||
| - | |<html><a name="invF">invF</a></html> | + | |<html><a name="invF">invF (-overhang)</a></html> |
|ATCTAGATTATATTGACAGCGCACAGAGCGGGAA | |ATCTAGATTATATTGACAGCGCACAGAGCGGGAA | ||
| - | |style="text-align: | + | |style="text-align: left;"|forward primer for [https://2009.igem.org/Team:Warsaw/Glossary#invasin invasin] |
|- | |- | ||
| - | |<html><a name="invR">invR</a></html> | + | |<html><a name="invR">invR (-overhang)</a></html> |
|GTACTAGTCATGGCTGGGGCTTCAGAAAAATATGAT | |GTACTAGTCATGGCTGGGGCTTCAGAAAAATATGAT | ||
| - | |style="text-align: | + | |style="text-align: left;"|reverse primer for [https://2009.igem.org/Team:Warsaw/Glossary#invasin invasin] |
| + | |- | ||
| + | |<html><a name="invF">invF (+overhang)</a></html> | ||
| + | |GAATTCGCGGCCGCTTCTAGATGTATTCATTTTTTAATACGCTAACTGT | ||
| + | |style="text-align: left;"|forward primer for [https://2009.igem.org/Team:Warsaw/Glossary#invasin invasin] | ||
| + | |- | ||
| + | |<html><a name="invR">invR (+overhang)</a></html> | ||
| + | |CTGCAGCGGCCGCTACTAGTACTATTGAGGCTCCGCACACAG | ||
| + | |style="text-align: left;"|reverse primer for [https://2009.igem.org/Team:Warsaw/Glossary#invasin invasin] | ||
|- | |- | ||
|<html><a name="hlyF">hlyF</a></html> | |<html><a name="hlyF">hlyF</a></html> | ||
|TGTCTAGAATGAAAAAAATAATGCTAGTT | |TGTCTAGAATGAAAAAAATAATGCTAGTT | ||
| - | |style="text-align: | + | |style="text-align: left;"|forward primer for [https://2009.igem.org/Team:Warsaw/Glossary#listeriolysin listeriolysin] |
|- | |- | ||
|<html><a name="hlyR">hlyR</a></html> | |<html><a name="hlyR">hlyR</a></html> | ||
|GACTAGTTTATTCGATTGGATTATCTA | |GACTAGTTTATTCGATTGGATTATCTA | ||
| - | |style="text-align: | + | |style="text-align: left;"|reverse primer for [https://2009.igem.org/Team:Warsaw/Glossary#listeriolysin listeriolysin] |
|- | |- | ||
|<html><a name="croF">croF</a></html> | |<html><a name="croF">croF</a></html> | ||
|ATCTAGAATGGAACAACGCATAACC | |ATCTAGAATGGAACAACGCATAACC | ||
| - | |style="text-align: | + | |style="text-align: left;"|forward primer for [https://2009.igem.org/Team:Warsaw/Glossary#cro cro] |
|- | |- | ||
|<html><a name="croR">croR</a></html> | |<html><a name="croR">croR</a></html> | ||
|GTACTAGTGGGCTGGAATGTGTAAG | |GTACTAGTGGGCTGGAATGTGTAAG | ||
| - | |style="text-align: | + | |style="text-align: left;"|reverse primer for [https://2009.igem.org/Team:Warsaw/Glossary#cro cro] |
|- | |- | ||
|<html><a name="phoF">phoF</a></html> | |<html><a name="phoF">phoF</a></html> | ||
|ATCTAGAATGATGCGCGTACTGGTTGTAG | |ATCTAGAATGATGCGCGTACTGGTTGTAG | ||
| - | |style="text-align: | + | |style="text-align: left;"|forward primer for [https://2009.igem.org/Team:Warsaw/Glossary#phoP.2FPhoQ phoP/phoQ] |
|- | |- | ||
|<html><a name="phoR">phoR</a></html> | |<html><a name="phoR">phoR</a></html> | ||
|TTACTAGTGATGCGTGGAAGAACGCACAG | |TTACTAGTGATGCGTGGAAGAACGCACAG | ||
| - | |style="text-align: | + | |style="text-align: left;"|reverse primer for [https://2009.igem.org/Team:Warsaw/Glossary#phoP.2FPhoQ PhoP/PhoQ] |
|- | |- | ||
|<html><a name="mgtCF">mgtCF</a></html> | |<html><a name="mgtCF">mgtCF</a></html> | ||
|ATCTAGATCCATTTTTTCTGGAAGAATAAG | |ATCTAGATCCATTTTTTCTGGAAGAATAAG | ||
| - | |style="text-align: | + | |style="text-align: left;"|forward primer for the [https://2009.igem.org/Team:Warsaw/Glossary#mgtc_gene_promoter mgtc gene promoter] |
|- | |- | ||
|<html><a name="mgtCR">mgtCR</a></html> | |<html><a name="mgtCR">mgtCR</a></html> | ||
|GTACTAGTCCTCGTCATGTTGTTTTATTTTTT | |GTACTAGTCCTCGTCATGTTGTTTTATTTTTT | ||
| - | |style="text-align: | + | |style="text-align: left;"|reverse primer for the [https://2009.igem.org/Team:Warsaw/Glossary#mgtc_gene_promoter mgtc gene promoter] |
|- | |- | ||
|<html><a name="p53F">p53F</a></html> | |<html><a name="p53F">p53F</a></html> | ||
|GCTCTAGAATGGAGGAGCCGCAGTC | |GCTCTAGAATGGAGGAGCCGCAGTC | ||
| - | |style="text-align: | + | |style="text-align: left;"|forward primer for [https://2009.igem.org/Team:Warsaw/Glossary#p53 p53] |
|- | |- | ||
|<html><a name="p53R">p53R</a> </html> | |<html><a name="p53R">p53R</a> </html> | ||
|GCACTAGTTCAGTCTGAGTCAGGCCCTTCTGT | |GCACTAGTTCAGTCTGAGTCAGGCCCTTCTGT | ||
| - | |style="text-align: | + | |style="text-align: left;"|reverse primer for [https://2009.igem.org/Team:Warsaw/Glossary#p53 p53] |
|- | |- | ||
|<html><a name="phoPF">phoPF</a> </html> | |<html><a name="phoPF">phoPF</a> </html> | ||
| - | | | + | |GTTTCTTCGAATTCGCGGCCGCTTCTAGATGATGCGCGTACTGGTTGTAGAG |
| - | |style="text-align: | + | |style="text-align: left;"|forward primer for [https://2009.igem.org/Team:Warsaw/Glossary#phoP.2FPhoQ PhoP] (new) |
|- | |- | ||
|<html><a name="phoPR">phoPR</a> </html> | |<html><a name="phoPR">phoPR</a> </html> | ||
| - | | | + | |GTTTCTTCCTGCAGCGGCCGCTACTAGTATTAGCGCAATTCAAAAAGATATCCTTGTCC |
| - | |style="text-align: | + | |style="text-align: left;"|reverse primer for [https://2009.igem.org/Team:Warsaw/Glossary#phoP.2FPhoQ PhoP] (new) |
|- | |- | ||
|<html><a name="phoQF">phoQF</a> </html> | |<html><a name="phoQF">phoQF</a> </html> | ||
| - | | | + | |GTTTCTTCGAATTCGCGGCCGCTTCTAGAGATGAATAAATTTGCTCGCCATTTTCTGC |
| - | |style="text-align: | + | |style="text-align: left;"|forward primer for [https://2009.igem.org/Team:Warsaw/Glossary#phoP.2FPhoQ PhoQ] (new) |
|- | |- | ||
|<html><a name="phoQR">phoQR</a> </html> | |<html><a name="phoQR">phoQR</a> </html> | ||
| - | | | + | |GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTCCTCTTTCTGTGTGGGATGCTGTC |
| - | |style="text-align: | + | |style="text-align: left;"|reverse primer for [https://2009.igem.org/Team:Warsaw/Glossary#phoP.2FPhoQ PhoQ] (new) |
|- | |- | ||
|<html><a name="baxF">baxF</a> </html> | |<html><a name="baxF">baxF</a> </html> | ||
|ATATAGAATTCCGCGGCCGCTTCTAGATGGACGGGTCCGGGGAG | |ATATAGAATTCCGCGGCCGCTTCTAGATGGACGGGTCCGGGGAG | ||
| - | |style="text-align: | + | |style="text-align: left;"|forward primer for [https://2009.igem.org/Team:Warsaw/Glossary#bax bax] |
|- | |- | ||
|<html><a name="baxR">baxR</a> </html> | |<html><a name="baxR">baxR</a> </html> | ||
|ACTGCTGCAGCGGCCGCTACTAGTATTAACCGGTTCAGCCCATCTTCTTCCAGATG | |ACTGCTGCAGCGGCCGCTACTAGTATTAACCGGTTCAGCCCATCTTCTTCCAGATG | ||
| - | |style="text-align: | + | |style="text-align: left;"|reverse primer for [https://2009.igem.org/Team:Warsaw/Glossary#bax bax] |
|- | |- | ||
|<html><a name="invF">invF</a> </html> | |<html><a name="invF">invF</a> </html> | ||
|GAATTCGCGGCCGCTTCTAGATGTATTCATTTTTTAATACGCTAACTGT | |GAATTCGCGGCCGCTTCTAGATGTATTCATTTTTTAATACGCTAACTGT | ||
| - | |style="text-align: | + | |style="text-align: left;"|forward primer for [https://2009.igem.org/Team:Warsaw/Glossary#invasin invasin] (with completely prefix and sufix) |
|- | |- | ||
|<html><a name="invR">invR</a> </html> | |<html><a name="invR">invR</a> </html> | ||
|TACTAGTAGCGGCCGCTGCAGCTATTGAGGCTCCGCACACAG | |TACTAGTAGCGGCCGCTGCAGCTATTGAGGCTCCGCACACAG | ||
| - | |style="text-align: | + | |style="text-align: left;"|reverse primer for [https://2009.igem.org/Team:Warsaw/Glossary#invasin invasin] (with completely prefix and sufix) |
|- | |- | ||
|<html><a name="inlapsF">inlapsF</a> </html> | |<html><a name="inlapsF">inlapsF</a> </html> | ||
|GCGCTGCAGTCACACAGGAAAGGTGAGAAAAAAACGATATG | |GCGCTGCAGTCACACAGGAAAGGTGAGAAAAAAACGATATG | ||
| - | |style="text-align: | + | |style="text-align: left;"|forward primer for [https://2009.igem.org/Team:Warsaw/Glossary#internalin internalin A] |
|- | |- | ||
|<html><a name="inlapsR">inlapsR</a></html> | |<html><a name="inlapsR">inlapsR</a></html> | ||
|GCGCTGCAGTTATTTACTAGCACGTGCTT | |GCGCTGCAGTTATTTACTAGCACGTGCTT | ||
| - | |style="text-align: | + | |style="text-align: left;"|reverse primer for [https://2009.igem.org/Team:Warsaw/Glossary#internalin internalin A] |
|- | |- | ||
|<html><a name="JPSwiF">JPSwiF</a></html> | |<html><a name="JPSwiF">JPSwiF</a></html> | ||
|TACTAGTAGCGGCCGCTGCAGCAATACGCAAACCGCCTCTC | |TACTAGTAGCGGCCGCTGCAGCAATACGCAAACCGCCTCTC | ||
| - | |style="text-align: | + | |style="text-align: left;"| |
|- | |- | ||
|<html><a name="JPSwiRbez">JPSwiRbez</a></html> | |<html><a name="JPSwiRbez">JPSwiRbez</a></html> | ||
|GAATTCGCGGCCGCTTCTAGAGTCACACTGGCTCACCTTC | |GAATTCGCGGCCGCTTCTAGAGTCACACTGGCTCACCTTC | ||
| - | |style="text-align: | + | |style="text-align: left;"| |
|- | |- | ||
|<html><a name="JPSwiRGFP">JPSwiRGFP</a></html> | |<html><a name="JPSwiRGFP">JPSwiRGFP</a></html> | ||
|GAATTCGCGGCCGCTTCTAGAGTATAAACGCAGAAAGG | |GAATTCGCGGCCGCTTCTAGAGTATAAACGCAGAAAGG | ||
| - | |style="text-align: | + | |style="text-align: left;"| |
| - | + | |- | |
| + | |<html><a name="mgtCnewF">mgtCnewF</a> </html> | ||
| + | |GAATTCGCGGCCGCTTCTAGAGTCCATTTTTTCTGGAAGAATAAG | ||
| + | |style="text-align: left;"|revised forward primer for the [https://2009.igem.org/Team:Warsaw/Glossary#mgtc_gene_promoter mgtc gene promoter] (now with complete prefix) | ||
| + | |- | ||
| + | |<html><a name="mgtCnewR">mgtCnewR</a></html> | ||
| + | |CTGCAGCGGCCGCTACTAGTACCTCGTCATGTTGTTTTATTTTTTT | ||
| + | |style="text-align: left;"|revised reverse primer for the [https://2009.igem.org/Team:Warsaw/Glossary#mgtc_gene_promoter mgtc gene promoter] (now with complete suffix) | ||
| + | |- | ||
| + | |<html><a name="YFPsilverF">YFPsilverF</a></html> | ||
| + | |GATGAATTCGCGGCCGCTTCTAGAATGGTGAGCAAGGGCGAG | ||
| + | |style="text-align: left;"|forward primer for <partinfo>E0032</partinfo> compatible with [http://dspace.mit.edu/handle/1721.1/32535 Silver Standard] | ||
| + | |- | ||
| + | |<html><a name="YFPsilverR">YFPsilverR</a></html> | ||
| + | |GCTCTGCAGCGGCCGCTACTAGTAGCTACTAAAGCGTAGTT | ||
| + | |style="text-align: left;"|reverse primer for <partinfo>E0032</partinfo> compatible with [http://dspace.mit.edu/handle/1721.1/32535 Silver Standard] | ||
| + | |- | ||
| + | |<html><a name="mitoCOXsilverF">mitoCOXsilverF</a></html> | ||
| + | |TTCGAATTCGCGGCCGCTTCTAGAATGTCCGTCCTGACGCCGCTG | ||
| + | |style="text-align: left;"|forward primer for <partinfo>K177028</partinfo> compatible with [http://dspace.mit.edu/handle/1721.1/32535 Silver Standard] | ||
| + | |- | ||
| + | |<html><a name="mitoCOXsilverR">mitoCOXsilverR</a></html> | ||
| + | |TTCCTGCAGCGGCCGCTACTAGTCAACGAATGGATCTTGGCGCGC | ||
| + | |style="text-align: left;"|reverse primer for <partinfo>K177028</partinfo> compatible with [http://dspace.mit.edu/handle/1721.1/32535 Silver Standard] | ||
| + | |- | ||
| + | |<html><a name="p53silverF">p53silverF</a></html> | ||
| + | |CGAGAATTCGCGGCCGCTTCTAGAATGGAGGAGCCGCAGTC | ||
| + | |style="text-align: left;"|forward primer for [https://2009.igem.org/Team:Warsaw/Glossary#p53 p53] CDS compatible with [http://dspace.mit.edu/handle/1721.1/32535 Silver Standard] | ||
| + | |- | ||
| + | |<html><a name="p53silverR">p53silverR</a></html> | ||
| + | |TTCGAATTCGCGGCCGCTTCTAGAATGTCCGTCCTGACGCCGCTG | ||
| + | |style="text-align: left;"|reverse primer for [https://2009.igem.org/Team:Warsaw/Glossary#p53 p53] CDS compatible with [http://dspace.mit.edu/handle/1721.1/32535 Silver Standard] | ||
| + | |- | ||
| + | |<html><a name="secr_silverF">secr_silverF</a></html> | ||
| + | |GGGAATTCCGCGGCCGCTTCTAGAGATGGCGGAGAAGGGAATG | ||
| + | |style="text-align: left;"|forward primer for [https://2009.igem.org/Team:Warsaw/Glossary#Type_I_secretion_system hemolysin secretion signal] compatible with [http://dspace.mit.edu/handle/1721.1/32535 Silver Standard] | ||
| + | |- | ||
| + | |<html><a name="secr_silverR">secr_silverR</a></html> | ||
| + | |CGGCTGCAGCGGCCGCTACTAGTTTATGCTGATGCTGTCAAAGTTATTG | ||
| + | |style="text-align: left;"|reverse primer for [https://2009.igem.org/Team:Warsaw/Glossary#Type_I_secretion_system hemolysin secretion signal] compatible with [http://dspace.mit.edu/handle/1721.1/32535 Silver Standard] | ||
| + | |- | ||
|} | |} | ||
| - | |||
{{WarFoot1}} | {{WarFoot1}} | ||
Latest revision as of 22:08, 20 October 2009
Primers
Here is the list of primers we have been using during our Project.
cloning primers
| name | sequence | description |
|---|---|---|
| croboxF | ATCTAGATACCTCTGGCGGTGATACTAGTGT | forward primer for cro-box |
| croboxR | ACACTAGTATCACCGCCAGAGGTATCTAGAT | reverse primer for cro-box |
| invF (-overhang) | ATCTAGATTATATTGACAGCGCACAGAGCGGGAA | forward primer for invasin |
| invR (-overhang) | GTACTAGTCATGGCTGGGGCTTCAGAAAAATATGAT | reverse primer for invasin |
| invF (+overhang) | GAATTCGCGGCCGCTTCTAGATGTATTCATTTTTTAATACGCTAACTGT | forward primer for invasin |
| invR (+overhang) | CTGCAGCGGCCGCTACTAGTACTATTGAGGCTCCGCACACAG | reverse primer for invasin |
| hlyF | TGTCTAGAATGAAAAAAATAATGCTAGTT | forward primer for listeriolysin |
| hlyR | GACTAGTTTATTCGATTGGATTATCTA | reverse primer for listeriolysin |
| croF | ATCTAGAATGGAACAACGCATAACC | forward primer for cro |
| croR | GTACTAGTGGGCTGGAATGTGTAAG | reverse primer for cro |
| phoF | ATCTAGAATGATGCGCGTACTGGTTGTAG | forward primer for phoP/phoQ |
| phoR | TTACTAGTGATGCGTGGAAGAACGCACAG | reverse primer for PhoP/PhoQ |
| mgtCF | ATCTAGATCCATTTTTTCTGGAAGAATAAG | forward primer for the mgtc gene promoter |
| mgtCR | GTACTAGTCCTCGTCATGTTGTTTTATTTTTT | reverse primer for the mgtc gene promoter |
| p53F | GCTCTAGAATGGAGGAGCCGCAGTC | forward primer for p53 |
| p53R | GCACTAGTTCAGTCTGAGTCAGGCCCTTCTGT | reverse primer for p53 |
| phoPF | GTTTCTTCGAATTCGCGGCCGCTTCTAGATGATGCGCGTACTGGTTGTAGAG | forward primer for PhoP (new) |
| phoPR | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTAGCGCAATTCAAAAAGATATCCTTGTCC | reverse primer for PhoP (new) |
| phoQF | GTTTCTTCGAATTCGCGGCCGCTTCTAGAGATGAATAAATTTGCTCGCCATTTTCTGC | forward primer for PhoQ (new) |
| phoQR | GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTCCTCTTTCTGTGTGGGATGCTGTC | reverse primer for PhoQ (new) |
| baxF | ATATAGAATTCCGCGGCCGCTTCTAGATGGACGGGTCCGGGGAG | forward primer for bax |
| baxR | ACTGCTGCAGCGGCCGCTACTAGTATTAACCGGTTCAGCCCATCTTCTTCCAGATG | reverse primer for bax |
| invF | GAATTCGCGGCCGCTTCTAGATGTATTCATTTTTTAATACGCTAACTGT | forward primer for invasin (with completely prefix and sufix) |
| invR | TACTAGTAGCGGCCGCTGCAGCTATTGAGGCTCCGCACACAG | reverse primer for invasin (with completely prefix and sufix) |
| inlapsF | GCGCTGCAGTCACACAGGAAAGGTGAGAAAAAAACGATATG | forward primer for internalin A |
| inlapsR | GCGCTGCAGTTATTTACTAGCACGTGCTT | reverse primer for internalin A |
| JPSwiF | TACTAGTAGCGGCCGCTGCAGCAATACGCAAACCGCCTCTC | |
| JPSwiRbez | GAATTCGCGGCCGCTTCTAGAGTCACACTGGCTCACCTTC | |
| JPSwiRGFP | GAATTCGCGGCCGCTTCTAGAGTATAAACGCAGAAAGG | |
| mgtCnewF | GAATTCGCGGCCGCTTCTAGAGTCCATTTTTTCTGGAAGAATAAG | revised forward primer for the mgtc gene promoter (now with complete prefix) |
| mgtCnewR | CTGCAGCGGCCGCTACTAGTACCTCGTCATGTTGTTTTATTTTTTT | revised reverse primer for the mgtc gene promoter (now with complete suffix) |
| YFPsilverF | GATGAATTCGCGGCCGCTTCTAGAATGGTGAGCAAGGGCGAG | forward primer for compatible with Silver Standard |
| YFPsilverR | GCTCTGCAGCGGCCGCTACTAGTAGCTACTAAAGCGTAGTT | reverse primer for compatible with Silver Standard |
| mitoCOXsilverF | TTCGAATTCGCGGCCGCTTCTAGAATGTCCGTCCTGACGCCGCTG | forward primer for compatible with Silver Standard |
| mitoCOXsilverR | TTCCTGCAGCGGCCGCTACTAGTCAACGAATGGATCTTGGCGCGC | reverse primer for compatible with Silver Standard |
| p53silverF | CGAGAATTCGCGGCCGCTTCTAGAATGGAGGAGCCGCAGTC | forward primer for p53 CDS compatible with Silver Standard |
| p53silverR | TTCGAATTCGCGGCCGCTTCTAGAATGTCCGTCCTGACGCCGCTG | reverse primer for p53 CDS compatible with Silver Standard |
| secr_silverF | GGGAATTCCGCGGCCGCTTCTAGAGATGGCGGAGAAGGGAATG | forward primer for hemolysin secretion signal compatible with Silver Standard |
| secr_silverR | CGGCTGCAGCGGCCGCTACTAGTTTATGCTGATGCTGTCAAAGTTATTG | reverse primer for hemolysin secretion signal compatible with Silver Standard |
