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Team:Warsaw/Primers
From 2009.igem.org
(Difference between revisions)
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==cloning primers== | ==cloning primers== | ||
| - | {|class="wikitable" style="border: 0px; margin: 3px; text-align: justify;" | + | {|class="wikitable" style="border: 0px; margin: 3px; text-align: justify; font-size: 9px" |
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!name | !name | ||
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|<html><a name="croboxR">croboxR</a></html> | |<html><a name="croboxR">croboxR</a></html> | ||
|ACACTAGTATCACCGCCAGAGGTATCTAGAT | |ACACTAGTATCACCGCCAGAGGTATCTAGAT | ||
| - | |style="text-align: center;"| | + | |style="text-align: center;"|reverse primer for crobox |
|- | |- | ||
|<html><a name="invF">invF</a></html> | |<html><a name="invF">invF</a></html> | ||
|ATCTAGATTATATTGACAGCGCACAGAGCGGGAA | |ATCTAGATTATATTGACAGCGCACAGAGCGGGAA | ||
| - | |style="text-align: center;"| | + | |style="text-align: center;"|forward primer for invasin |
|- | |- | ||
|<html><a name="invR">invR</a></html> | |<html><a name="invR">invR</a></html> | ||
|GTACTAGTCATGGCTGGGGCTTCAGAAAAATATGAT | |GTACTAGTCATGGCTGGGGCTTCAGAAAAATATGAT | ||
| - | |style="text-align: center;"| | + | |style="text-align: center;"|reverse primer for invasin |
|- | |- | ||
|<html><a name="hlyF">hlyF</a></html> | |<html><a name="hlyF">hlyF</a></html> | ||
|TGTCTAGAATGAAAAAAATAATGCTAGTT | |TGTCTAGAATGAAAAAAATAATGCTAGTT | ||
| - | |style="text-align: center;"| | + | |style="text-align: center;"|forward primer for listeriolysin |
|- | |- | ||
|<html><a name="hlyR">hlyR</a></html> | |<html><a name="hlyR">hlyR</a></html> | ||
|GACTAGTTTATTCGATTGGATTATCTA | |GACTAGTTTATTCGATTGGATTATCTA | ||
| - | |style="text-align: center;"| | + | |style="text-align: center;"|reverse primer for listeriolysin |
|- | |- | ||
|<html><a name="croF">croF</a></html> | |<html><a name="croF">croF</a></html> | ||
|ATCTAGAATGGAACAACGCATAACC | |ATCTAGAATGGAACAACGCATAACC | ||
| - | |style="text-align: center;"| | + | |style="text-align: center;"|forward primer for cro |
|- | |- | ||
|<html><a name="croR">croR</a></html> | |<html><a name="croR">croR</a></html> | ||
|GTACTAGTGGGCTGGAATGTGTAAG | |GTACTAGTGGGCTGGAATGTGTAAG | ||
| - | |style="text-align: center;"| | + | |style="text-align: center;"|reverse primer for cro |
|- | |- | ||
|<html><a name="phoF">phoF</a></html> | |<html><a name="phoF">phoF</a></html> | ||
|ATCTAGAATGATGCGCGTACTGGTTGTAG | |ATCTAGAATGATGCGCGTACTGGTTGTAG | ||
| - | |style="text-align: center;"| | + | |style="text-align: center;"|forward primer for phoP/phoQ |
|- | |- | ||
|<html><a name="phoR">phoR</a></html> | |<html><a name="phoR">phoR</a></html> | ||
|TTACTAGTGATGCGTGGAAGAACGCACAG | |TTACTAGTGATGCGTGGAAGAACGCACAG | ||
| - | |style="text-align: center;"| | + | |style="text-align: center;"|reverse primer for phoP/phoQ |
|- | |- | ||
|<html><a name="mgtCF">mgtCF</a></html> | |<html><a name="mgtCF">mgtCF</a></html> | ||
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|<html><a name="p53F">p53F</a></html> | |<html><a name="p53F">p53F</a></html> | ||
|GCTCTAGAATGGAGGAGCCGCAGTC | |GCTCTAGAATGGAGGAGCCGCAGTC | ||
| - | |style="text-align: center;"| | + | |style="text-align: center;"|forward primer for p53 |
|- | |- | ||
|<html><a name="p53R">p53R</a> </html> | |<html><a name="p53R">p53R</a> </html> | ||
|GCACTAGTTCAGTCTGAGTCAGGCCCTTCTGT | |GCACTAGTTCAGTCTGAGTCAGGCCCTTCTGT | ||
| - | |style="text-align: center;"| | + | |style="text-align: center;"|reverse primer for p53 |
|- | |- | ||
|<html><a name="phoPF">phoPF</a> </html> | |<html><a name="phoPF">phoPF</a> </html> | ||
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|<html><a name="phoPR">phoPR</a> </html> | |<html><a name="phoPR">phoPR</a> </html> | ||
|gtttcttcctgcagcggccgctactagtattagcgcaattcaaaaagatatccttgtcc | |gtttcttcctgcagcggccgctactagtattagcgcaattcaaaaagatatccttgtcc | ||
| - | |style="text-align: center;"| | + | |style="text-align: center;"|reverse primer for phoP (new) |
|- | |- | ||
|<html><a name="phoQF">phoQF</a> </html> | |<html><a name="phoQF">phoQF</a> </html> | ||
|gtttcttcgaattcgcggccgcttctagagatgaataaatttgctcgccattttctgcc | |gtttcttcgaattcgcggccgcttctagagatgaataaatttgctcgccattttctgcc | ||
| - | |style="text-align: center;"| | + | |style="text-align: center;"|forward primer for phoQ (new) |
|- | |- | ||
|<html><a name="phoQR">phoQR</a> </html> | |<html><a name="phoQR">phoQR</a> </html> | ||
|gtttcttcctgcagcggccgctactagtattattcctctttctgtgtgggatgctg | |gtttcttcctgcagcggccgctactagtattattcctctttctgtgtgggatgctg | ||
| - | |style="text-align: center;"| | + | |style="text-align: center;"|reverse primer for phoP (new) |
|- | |- | ||
|<html><a name="baxF">baxF</a> </html> | |<html><a name="baxF">baxF</a> </html> | ||
|ATATAGAATTCCGCGGCCGCTTCTAGATGGACGGGTCCGGGGAG | |ATATAGAATTCCGCGGCCGCTTCTAGATGGACGGGTCCGGGGAG | ||
| - | |style="text-align: center;"| | + | |style="text-align: center;"|forward primer for bax |
|- | |- | ||
|<html><a name="baxR">baxR</a> </html> | |<html><a name="baxR">baxR</a> </html> | ||
|ACTGCTGCAGCGGCCGCTACTAGTATTAACCGGTTCAGCCCATCTTCTTCCAGATG | |ACTGCTGCAGCGGCCGCTACTAGTATTAACCGGTTCAGCCCATCTTCTTCCAGATG | ||
| - | |style="text-align: center;"| | + | |style="text-align: center;"|reverse primer for bax |
|- | |- | ||
|<html><a name="invF">invF</a> </html> | |<html><a name="invF">invF</a> </html> | ||
|GAATTCGCGGCCGCTTCTAGATGTATTCATTTTTTAATACGCTAACTGT | |GAATTCGCGGCCGCTTCTAGATGTATTCATTTTTTAATACGCTAACTGT | ||
| - | |style="text-align: center;"| | + | |style="text-align: center;"|forward primer for invasin (with completely prefix and sufix) |
|- | |- | ||
|<html><a name="invR">invR</a> </html> | |<html><a name="invR">invR</a> </html> | ||
|TACTAGTAGCGGCCGCTGCAGCTATTGAGGCTCCGCACACAG | |TACTAGTAGCGGCCGCTGCAGCTATTGAGGCTCCGCACACAG | ||
| - | |style="text-align: center;"| | + | |style="text-align: center;"|reverse primer for invasin (with completely prefix and sufix) |
|- | |- | ||
|<html><a name="inlapsF">inlapsF</a> </html> | |<html><a name="inlapsF">inlapsF</a> </html> | ||
Revision as of 22:24, 15 September 2009
Primers
Here is the list of primers we have been using during our Project.
cloning primers
| name | sequence | description |
|---|---|---|
| croboxF | ATCTAGATACCTCTGGCGGTGATACTAGTGT | forward primer for crobox |
| croboxR | ACACTAGTATCACCGCCAGAGGTATCTAGAT | reverse primer for crobox |
| invF | ATCTAGATTATATTGACAGCGCACAGAGCGGGAA | forward primer for invasin |
| invR | GTACTAGTCATGGCTGGGGCTTCAGAAAAATATGAT | reverse primer for invasin |
| hlyF | TGTCTAGAATGAAAAAAATAATGCTAGTT | forward primer for listeriolysin |
| hlyR | GACTAGTTTATTCGATTGGATTATCTA | reverse primer for listeriolysin |
| croF | ATCTAGAATGGAACAACGCATAACC | forward primer for cro |
| croR | GTACTAGTGGGCTGGAATGTGTAAG | reverse primer for cro |
| phoF | ATCTAGAATGATGCGCGTACTGGTTGTAG | forward primer for phoP/phoQ |
| phoR | TTACTAGTGATGCGTGGAAGAACGCACAG | reverse primer for phoP/phoQ |
| mgtCF | ATCTAGATCCATTTTTTCTGGAAGAATAAG | forward primer for the mgtc gene promoter |
| mgtCR | GTACTAGTCCTCGTCATGTTGTTTTATTTTTT | reverse primer for the mgtc gene promoter |
| p53F | GCTCTAGAATGGAGGAGCCGCAGTC | forward primer for p53 |
| p53R | GCACTAGTTCAGTCTGAGTCAGGCCCTTCTGT | reverse primer for p53 |
| phoPF | gtttcttcgaattcgcggccgcttctagatgatgcgcgtactggttgtagag | |
| phoPR | gtttcttcctgcagcggccgctactagtattagcgcaattcaaaaagatatccttgtcc | reverse primer for phoP (new) |
| phoQF | gtttcttcgaattcgcggccgcttctagagatgaataaatttgctcgccattttctgcc | forward primer for phoQ (new) |
| phoQR | gtttcttcctgcagcggccgctactagtattattcctctttctgtgtgggatgctg | reverse primer for phoP (new) |
| baxF | ATATAGAATTCCGCGGCCGCTTCTAGATGGACGGGTCCGGGGAG | forward primer for bax |
| baxR | ACTGCTGCAGCGGCCGCTACTAGTATTAACCGGTTCAGCCCATCTTCTTCCAGATG | reverse primer for bax |
| invF | GAATTCGCGGCCGCTTCTAGATGTATTCATTTTTTAATACGCTAACTGT | forward primer for invasin (with completely prefix and sufix) |
| invR | TACTAGTAGCGGCCGCTGCAGCTATTGAGGCTCCGCACACAG | reverse primer for invasin (with completely prefix and sufix) |
| inlapsF | GCGCTGCAGTCACACAGGAAAGGTGAGAAAAAAACGATATG | forward primer for internalinA |
| inlapsR | GCGCTGCAGTTATTTACTAGCACGTGCTT | reverse primer for internalinA |
| JPSwiF | TACTAGTAGCGGCCGCTGCAGCAATACGCAAACCGCCTCTC | |
| JPSwiRbez | GAATTCGCGGCCGCTTCTAGAGTCACACTGGCTCACCTTC | |
| JPSwiRGFP | GAATTCGCGGCCGCTTCTAGAGTATAAACGCAGAAAGG |
