Team:Warsaw/Primers

From 2009.igem.org

(Difference between revisions)
Line 26: Line 26:
<a name="p53F">p53F</a>      5' GCTCTAGAATGGAGGAGCCGCAGTC  3'
<a name="p53F">p53F</a>      5' GCTCTAGAATGGAGGAGCCGCAGTC  3'
<a name="p53R">p53R</a>      5' GCACTAGTTCAGTCTGAGTCAGGCCCTTCTGT  3'
<a name="p53R">p53R</a>      5' GCACTAGTTCAGTCTGAGTCAGGCCCTTCTGT  3'
-
<a name="phoPF">phoPF</a>     5' gtttcttcgaattcgcggccgcttctagatgatgcgcgtactggttgtagag  3'
+
<a name="phoPF">phoPF</a>     5' gtttcttcgaattcgcggccgcttctagatgatgcgcgtactggttgtagag  3'
-
<a name="phoPR">phoPR</a>     5' gtttcttcctgcagcggccgctactagtattagcgcaattcaaaaagatatccttgtcc  3'
+
<a name="phoPR">phoPR</a>     5' gtttcttcctgcagcggccgctactagtattagcgcaattcaaaaagatatccttgtcc  3'
-
<a name="phoQF">phoQF</a>     5' gtttcttcgaattcgcggccgcttctagagatgaataaatttgctcgccattttctgcc  3'
+
<a name="phoQF">phoQF</a>     5' gtttcttcgaattcgcggccgcttctagagatgaataaatttgctcgccattttctgcc  3'
-
<a name="phoQR">phoQR</a>     5' gtttcttcctgcagcggccgctactagtattattcctctttctgtgtgggatgctg  3'
+
<a name="phoQR">phoQR</a>     5' gtttcttcctgcagcggccgctactagtattattcctctttctgtgtgggatgctg  3'
 +
<a name="baxF">baxF</a>      5' ATATAGAATTCCGCGGCCGCTTCTAGATGGACGGGTCCGGGGAG 3'
 +
<a name="baxR">baxR</a>      5' ACTGCTGCAGCGGCCGCTACTAGTATTAACCGGTTCAGCCCATCTTCTTCCAGATG 3'
 +
<a name="invF">invF</a>      5' GAATTCGCGGCCGCTTCTAGATGTATTCATTTTTTAATACGCTAACTGT 3'
 +
<a name="invR">invR</a>      5' TACTAGTAGCGGCCGCTGCAGCTATTGAGGCTCCGCACACAG 3'
 +
<a name="inlapsF">inlapsF</a>  5' GCGCTGCAGTCACACAGGAAAGGTGAGAAAAAAACGATATG 3'
 +
<a name="inlapsR">inlapsR</a>  5' GCGCTGCAGTTATTTACTAGCACGTGCTT 3'
</pre>
</pre>

Revision as of 11:10, 15 September 2009


Primers

cloning primers

cloning primers

croboxF   5' ATCTAGATACCTCTGGCGGTGATACTAGTGT  3'
croboxR   5' ACACTAGTATCACCGCCAGAGGTATCTAGAT  3'
invF      5' ATCTAGATTATATTGACAGCGCACAGAGCGGGAA  3'
invR      5' GTACTAGTCATGGCTGGGGCTTCAGAAAAATATGAT  3'
hlyF      5' TGTCTAGAATGAAAAAAATAATGCTAGTT  3'
hlyR      5' GACTAGTTTATTCGATTGGATTATCTA  3'
croF      5' ATCTAGAATGGAACAACGCATAACC  3'
croR      5' GTACTAGTGGGCTGGAATGTGTAAG  3'
phoF      5' ATCTAGAATGATGCGCGTACTGGTTGTAG  3'
phoR      5' TTACTAGTGATGCGTGGAAGAACGCACAG  3'
mgtCF     5' ATCTAGATCCATTTTTTCTGGAAGAATAAG  3'
mgtCR     5' GTACTAGTCCTCGTCATGTTGTTTTATTTTTT  3'
p53F      5' GCTCTAGAATGGAGGAGCCGCAGTC  3'
p53R      5' GCACTAGTTCAGTCTGAGTCAGGCCCTTCTGT  3'
phoPF     5' gtttcttcgaattcgcggccgcttctagatgatgcgcgtactggttgtagag  3'
phoPR     5' gtttcttcctgcagcggccgctactagtattagcgcaattcaaaaagatatccttgtcc  3'
phoQF     5' gtttcttcgaattcgcggccgcttctagagatgaataaatttgctcgccattttctgcc  3'
phoQR     5' gtttcttcctgcagcggccgctactagtattattcctctttctgtgtgggatgctg  3'
baxF      5' ATATAGAATTCCGCGGCCGCTTCTAGATGGACGGGTCCGGGGAG 3'
baxR      5' ACTGCTGCAGCGGCCGCTACTAGTATTAACCGGTTCAGCCCATCTTCTTCCAGATG 3'
invF      5' GAATTCGCGGCCGCTTCTAGATGTATTCATTTTTTAATACGCTAACTGT 3'
invR      5' TACTAGTAGCGGCCGCTGCAGCTATTGAGGCTCCGCACACAG 3'
inlapsF   5' GCGCTGCAGTCACACAGGAAAGGTGAGAAAAAAACGATATG 3'
inlapsR   5' GCGCTGCAGTTATTTACTAGCACGTGCTT 3'


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