Team:Warsaw/Primers

From 2009.igem.org

Primers

Here is the list of primers we have been using during our Project.

cloning primers

name sequence description
croboxF ATCTAGATACCTCTGGCGGTGATACTAGTGT forward primer for cro-box
croboxR ACACTAGTATCACCGCCAGAGGTATCTAGAT reverse primer for cro-box
invF (-overhang) ATCTAGATTATATTGACAGCGCACAGAGCGGGAA forward primer for invasin
invR (-overhang) GTACTAGTCATGGCTGGGGCTTCAGAAAAATATGAT reverse primer for invasin
invF (+overhang) GAATTCGCGGCCGCTTCTAGATGTATTCATTTTTTAATACGCTAACTGT forward primer for invasin
invR (+overhang) CTGCAGCGGCCGCTACTAGTACTATTGAGGCTCCGCACACAG reverse primer for invasin
hlyF TGTCTAGAATGAAAAAAATAATGCTAGTT forward primer for listeriolysin
hlyR GACTAGTTTATTCGATTGGATTATCTA reverse primer for listeriolysin
croF ATCTAGAATGGAACAACGCATAACC forward primer for cro
croR GTACTAGTGGGCTGGAATGTGTAAG reverse primer for cro
phoF ATCTAGAATGATGCGCGTACTGGTTGTAG forward primer for phoP/phoQ
phoR TTACTAGTGATGCGTGGAAGAACGCACAG reverse primer for PhoP/PhoQ
mgtCF ATCTAGATCCATTTTTTCTGGAAGAATAAG forward primer for the mgtc gene promoter
mgtCR GTACTAGTCCTCGTCATGTTGTTTTATTTTTT reverse primer for the mgtc gene promoter
p53F GCTCTAGAATGGAGGAGCCGCAGTC forward primer for p53
p53R GCACTAGTTCAGTCTGAGTCAGGCCCTTCTGT reverse primer for p53
phoPF GTTTCTTCGAATTCGCGGCCGCTTCTAGATGATGCGCGTACTGGTTGTAGAG forward primer for PhoP (new)
phoPR GTTTCTTCCTGCAGCGGCCGCTACTAGTATTAGCGCAATTCAAAAAGATATCCTTGTCC reverse primer for PhoP (new)
phoQF GTTTCTTCGAATTCGCGGCCGCTTCTAGAGATGAATAAATTTGCTCGCCATTTTCTGC forward primer for PhoQ (new)
phoQR GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATTCCTCTTTCTGTGTGGGATGCTGTC reverse primer for PhoQ (new)
baxF ATATAGAATTCCGCGGCCGCTTCTAGATGGACGGGTCCGGGGAG forward primer for bax
baxR ACTGCTGCAGCGGCCGCTACTAGTATTAACCGGTTCAGCCCATCTTCTTCCAGATG reverse primer for bax
invF GAATTCGCGGCCGCTTCTAGATGTATTCATTTTTTAATACGCTAACTGT forward primer for invasin (with completely prefix and sufix)
invR TACTAGTAGCGGCCGCTGCAGCTATTGAGGCTCCGCACACAG reverse primer for invasin (with completely prefix and sufix)
inlapsF GCGCTGCAGTCACACAGGAAAGGTGAGAAAAAAACGATATG forward primer for internalin A
inlapsR GCGCTGCAGTTATTTACTAGCACGTGCTT reverse primer for internalin A
JPSwiF TACTAGTAGCGGCCGCTGCAGCAATACGCAAACCGCCTCTC
JPSwiRbez GAATTCGCGGCCGCTTCTAGAGTCACACTGGCTCACCTTC
JPSwiRGFP GAATTCGCGGCCGCTTCTAGAGTATAAACGCAGAAAGG
mgtCnewF GAATTCGCGGCCGCTTCTAGAGTCCATTTTTTCTGGAAGAATAAG revised forward primer for the mgtc gene promoter (now with complete prefix)
mgtCnewR CTGCAGCGGCCGCTACTAGTACCTCGTCATGTTGTTTTATTTTTTT revised reverse primer for the mgtc gene promoter (now with complete suffix)
YFPsilverF GATGAATTCGCGGCCGCTTCTAGAATGGTGAGCAAGGGCGAG forward primer for compatible with [http://dspace.mit.edu/handle/1721.1/32535 Silver Standard]
YFPsilverR GCTCTGCAGCGGCCGCTACTAGTAGCTACTAAAGCGTAGTT reverse primer for compatible with [http://dspace.mit.edu/handle/1721.1/32535 Silver Standard]
mitoCOXsilverF TTCGAATTCGCGGCCGCTTCTAGAATGTCCGTCCTGACGCCGCTG forward primer for compatible with [http://dspace.mit.edu/handle/1721.1/32535 Silver Standard]
mitoCOXsilverR TTCCTGCAGCGGCCGCTACTAGTCAACGAATGGATCTTGGCGCGC reverse primer for compatible with [http://dspace.mit.edu/handle/1721.1/32535 Silver Standard]
p53silverF CGAGAATTCGCGGCCGCTTCTAGAATGGAGGAGCCGCAGTC forward primer for p53 CDS compatible with [http://dspace.mit.edu/handle/1721.1/32535 Silver Standard]
p53silverR TTCGAATTCGCGGCCGCTTCTAGAATGTCCGTCCTGACGCCGCTG reverse primer for p53 CDS compatible with [http://dspace.mit.edu/handle/1721.1/32535 Silver Standard]
secr_silverF GGGAATTCCGCGGCCGCTTCTAGAGATGGCGGAGAAGGGAATG forward primer for hemolysin secretion signal compatible with [http://dspace.mit.edu/handle/1721.1/32535 Silver Standard]
secr_silverR CGGCTGCAGCGGCCGCTACTAGTTTATGCTGATGCTGTCAAAGTTATTG reverse primer for hemolysin secretion signal compatible with [http://dspace.mit.edu/handle/1721.1/32535 Silver Standard]