Team:Warsaw/Sources

From 2009.igem.org

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Most of bricks connected with bacteria invasiveness were acquired from following organisms:
Most of bricks connected with bacteria invasiveness were acquired from following organisms:
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{|class="wikitable" style="border: 0px; margin: 3px; text-align: justify;"  
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{|class="wikitable" style="border: 0px; margin: 3px; text-align: justify;" width="100%"
|-
|-
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!style="text-align: center"|organism
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!style="text-align: center" width="45%"|organism
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!style="text-align: center"|genomic sequence details
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!style="text-align: center" width="30%"|genomic sequence details
!style="text-align: center"|acquired genes
!style="text-align: center"|acquired genes
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|-
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|''Salmonella enterolitica'' ser. ''typhimurium'' LT2
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|''Salmonella enterolitica'' ser. ''typhimurium'' LT2<sup>1</sup>
|style="text-align: center;"|[http://www.ncbi.nlm.nih.gov/nuccore/NC_003197 NC_OO3197]
|style="text-align: center;"|[http://www.ncbi.nlm.nih.gov/nuccore/NC_003197 NC_OO3197]
|[https://2009.igem.org/Team:Warsaw/Glossary#phoP.2FPhoQ PhoP, PhoQ], [https://2009.igem.org/Team:Warsaw/Glossary#mgtc_gene_promoter MgtC promoter]
|[https://2009.igem.org/Team:Warsaw/Glossary#phoP.2FPhoQ PhoP, PhoQ], [https://2009.igem.org/Team:Warsaw/Glossary#mgtc_gene_promoter MgtC promoter]
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|-
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|''Listeria monocytogenes'' EGD-E
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|''Listeria monocytogenes'' EGD-E<sup>2</sup>
|style="text-align: center;"|[http://www.ncbi.nlm.nih.gov/nuccore/NC_003210 NC_003210]
|style="text-align: center;"|[http://www.ncbi.nlm.nih.gov/nuccore/NC_003210 NC_003210]
|[https://2009.igem.org/Team:Warsaw/Glossary#listeriolysin listeriolysin], [https://2009.igem.org/Team:Warsaw/Glossary#internalin internalinA]
|[https://2009.igem.org/Team:Warsaw/Glossary#listeriolysin listeriolysin], [https://2009.igem.org/Team:Warsaw/Glossary#internalin internalinA]
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|-
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|''Yersina enterolitica'' O:8 (8081)
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|''Yersina enterolitica'' O:8 (8081)<sup>3</sup>
|style="text-align: center;"|[http://www.ncbi.nlm.nih.gov/nuccore/AM286415 AM286415]
|style="text-align: center;"|[http://www.ncbi.nlm.nih.gov/nuccore/AM286415 AM286415]
|[https://2009.igem.org/Team:Warsaw/Glossary#invasin invasin]
|[https://2009.igem.org/Team:Warsaw/Glossary#invasin invasin]
|}
|}
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==Other bricks==
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{|class="wikitable" style="border: 0px; margin: 3px; text-align: justify;" width="100%"
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|-
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!style="text-align: center" width="45%"|name of the brick
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!style="text-align: center" width="30%"|sequence details
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!style="text-align: center"|source
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|-
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|style="text-align: center"|[https://2009.igem.org/Team:Warsaw/Glossary#p53 p53]
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|style="text-align: center"|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K177016 BBa_K177016]
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|human<sup>4</sup>
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|-
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|style="text-align: center"|[https://2009.igem.org/Team:Warsaw/Glossary#bax bax]
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|style="text-align: center"|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K177027 BBa_K177027]
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|human <sup>5</sup>
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|-
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|style="text-align: center"|mitochondrial targeting signal from the [https://2009.igem.org/Team:Warsaw/Glossary#cytochrome_C_oxidase Cox] gene
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|style="text-align: center"|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K177028 BBa_K177028]
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|human<sup>6</sup>
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|-
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|style="text-align: center"|secretion system
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|style="text-align: center"|
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|''Escherichia coli''<sup>7</sup>
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|}
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<sup>1</sup> Genomic DNA from this strain was a generous gift from ...
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<sup>2</sup> Genomic DNA from this strain was a generous gift from prof. Jacek Bielecki, PhD, [http://www.biol.uw.edu.pl/_2008eng/index_2501.php?a_2501=528&b_2501=5 Laboratory of Applied Microbiology], Faculty of Biology, University of Warsaw
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<sup>3</sup> Genomic DNA from this strain was a generous gift from ...
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<sup>4</sup> Plasmid carrying p53 gene was a generous gift from Dawid Walerych, PhD, from the [http://www.iimcb.gov.pl/zylicz_lab.php Lab of Molecular Biology], International Institute of Molecular and Cell Biology in Warsaw
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<sup>5</sup> Plasmid carrying bax gene was a generous gift from Łukasz Borowski, MSc, from the [http://www.igib.uw.edu.pl/index.php?id=80 Institute of Genetics and Biotechnology], University of Warsaw.
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<sup>6</sup> Mitochondrial targeting signal was amplified from the [http://www.clontech.com/images/pt/PT3730-5.pdf pAcGFP-mito] vector
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<sup>7</sup> Plasmids carrying essential elements of mentioned secretion system were a generous gift from ...
{{WarFoot1}}
{{WarFoot1}}

Revision as of 13:35, 15 September 2009

Sources of natural bricks

Below we present sources of bricks prepared by our team.

Invasiveness bricks

Most of bricks connected with bacteria invasiveness were acquired from following organisms:

organism genomic sequence details acquired genes
Salmonella enterolitica ser. typhimurium LT21 NC_OO3197 PhoP, PhoQ, MgtC promoter
Listeria monocytogenes EGD-E2 NC_003210 listeriolysin, internalinA
Yersina enterolitica O:8 (8081)3 AM286415 invasin

Other bricks

name of the brick sequence details source
p53 BBa_K177016 human4
bax BBa_K177027 human 5
mitochondrial targeting signal from the Cox gene BBa_K177028 human6
secretion system Escherichia coli7


1 Genomic DNA from this strain was a generous gift from ...

2 Genomic DNA from this strain was a generous gift from prof. Jacek Bielecki, PhD, Laboratory of Applied Microbiology, Faculty of Biology, University of Warsaw

3 Genomic DNA from this strain was a generous gift from ...

4 Plasmid carrying p53 gene was a generous gift from Dawid Walerych, PhD, from the Lab of Molecular Biology, International Institute of Molecular and Cell Biology in Warsaw

5 Plasmid carrying bax gene was a generous gift from Łukasz Borowski, MSc, from the Institute of Genetics and Biotechnology, University of Warsaw.

6 Mitochondrial targeting signal was amplified from the pAcGFP-mito vector

7 Plasmids carrying essential elements of mentioned secretion system were a generous gift from ...


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