Template:Team:KULeuven/2 September 2009/VanillinProduction

From 2009.igem.org

(Difference between revisions)
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** Overnight in 16C
** Overnight in 16C
* the plates with sam5, sam8, ech and fcs were re-ented. from sam5 and fcs for colonies were picked and plated out.
* the plates with sam5, sam8, ech and fcs were re-ented. from sam5 and fcs for colonies were picked and plated out.
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 +
* The PCR from yesterday did not deliver any results, probably because of an error when preparing the PCR mix, a new PCR was prepared and started today.

Revision as of 07:21, 3 September 2009

  • The 4 grown colonies are put on liquid culture
  • New (again...) restriction digest on Sam5, Sam8, Fcs and Ech, genes were mini prepped on tuesday.
    • Overnight in 16C
  • the plates with sam5, sam8, ech and fcs were re-ented. from sam5 and fcs for colonies were picked and plated out.
  • The PCR from yesterday did not deliver any results, probably because of an error when preparing the PCR mix, a new PCR was prepared and started today.