Revision as of 10:10, 10 September 2009

Gel extraction performed on the signals from 3/09
- Nanodrop: 14,7 ng/µl of with a 260/280 of approx 2,12
Ligation with and (ligX 1)
A new restriction digest on and with EcoRI and SpeI was performed.
Gel extraction of these new digests. was nanodropped with a concentration of 28 ng/µl. this was ligated with (ligX 2)
Electroporation of pSB3K3

Saturday

There was good growth of the cells that were electroporated with ligX. 4 single colonies were selected and plated on Ap medium. This was all done in a dark room to avoid activation of the promotor.

@@ Line 3: / Line 3: @@
 # Ligation with {{kulpart|BBa_J61002}} and {{kulpart|BBa_K238013}} (ligX 1)
 # A new restriction digest on {{kulpart|BBa_J61002}} and {{kulpart|BBa_K238013}} with EcoRI and SpeI was performed.
-# gel extraction of these new digests. {{kulpart|BBa_J61002}} was nanodropped with a concentration of 28 ng/µl. this was ligated with {{kulpart|BBa_K238013}} (ligX 2)
+# Gel extraction of these new digests. {{kulpart|BBa_J61002}} was nanodropped with a concentration of 28 ng/µl. this was ligated with {{kulpart|BBa_K238013}} (ligX 2)
-# electroporatie van pSB3K3
+# Electroporation of pSB3K3
-=saterday=
+=Saturday=
-#electroporation of ligX 1&2
+#Electroporation of ligX 1&2
-#there was no growth of cells after electroporation with pSB3K3
+#There was no growth of cells after electroporation with pSB3K3
-=sunday=
+=Sunday=
-# there was good growth of the cells that were electroporated with ligX. 4 single colonies were selected and plated on Ap medium. this was all done in a dark room to avoid activation of the promotor.
+# There was good growth of the cells that were electroporated with ligX. 4 single colonies were selected and plated on Ap medium. This was all done in a dark room to avoid activation of the promotor.