Uppsala-Sweden/10 October 2009

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Plasmid prep

A plasmid prep was performed for PRPRYT 3 & 4 on pSB1AK3.

Triparental mating

BG-11 plates were made without antibiotics and without CuSO4 to have the pPetE promoter as silent as possible during triparental mating.

Evaluate constructs in e.coli

The constructs were assembled with the lac promoter (R0011) to make it possible to evaluate them in e.coli, transformed into M15 carrying the lac repressor making it possible for us to induce with IPTG.




Bioneer Biolegio Clontech Uppsala Genome Center

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