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Uppsala-Sweden/8 October 2009
From 2009.igem.org
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(New page: {{Uppsala-Sweden Template}} ==Evaluating transformation== Colonies were picked for all the antisense RNA constructs and were evaluated on a gel. with the result that all constructs were ...) |
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The conjugation plasmi carrying strain was inoculated in preparation for triparental mating. | The conjugation plasmi carrying strain was inoculated in preparation for triparental mating. | ||
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| + | ==Transformation== | ||
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| + | Yesterdays digestions of RPRAT, RPRYT, PRKRYT were transformed. pPetE + RPRAT, pPetE + RPRYT and PRKRYT were moved to AC in preparation of triparental mating, since the carrier plasmid in only available with kanamycin resistance. | ||
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Latest revision as of 15:41, 21 October 2009
Evaluating transformation
Colonies were picked for all the antisense RNA constructs and were evaluated on a gel. with the result that all constructs were present in some colonies carrying the correct construct except E1B3.
Triparental mating
The conjugation plasmi carrying strain was inoculated in preparation for triparental mating.
Transformation
Yesterdays digestions of RPRAT, RPRYT, PRKRYT were transformed. pPetE + RPRAT, pPetE + RPRYT and PRKRYT were moved to AC in preparation of triparental mating, since the carrier plasmid in only available with kanamycin resistance.
