Virginia Commonwealth/11 June 2009
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==Thursday 11 June 2009== | ==Thursday 11 June 2009== | ||
===Results=== | ===Results=== | ||
- | *1 | + | *Note these were plated Wednesday evening with two groups splitting the work. |
- | *2 | + | *Group 1: Craig, Clay, Afton plates |
+ | *Observations on the Controls | ||
+ | |||
+ | {| cellpadding="5" border="1" padding="5" | ||
+ | |+ | ||
+ | |- | ||
+ | ! Control !! Plate Type !! Observation !! | ||
+ | |- | ||
+ | ! NEB10β (+) | ||
+ | | LB || lawn || | ||
+ | |- | ||
+ | ! NEB10β (-) | ||
+ | | Cm || no growth || | ||
+ | |- | ||
+ | ! NEB10β (-) | ||
+ | | Amp || no growth || | ||
+ | |- | ||
+ | ! NEB10β (-) | ||
+ | | Kan || 3 colonies || | ||
+ | |} | ||
+ | * Growth on Kan could have resulted from a non-homogeneous medium with very small sections of the plate being able to sustain life. Or there was degradation of the Kan-selective medium. | ||
+ | |||
+ | *Observations on the Colonies | ||
+ | {| cellpadding="5" border="1" padding="5" | ||
+ | |+ | ||
+ | |- | ||
+ | ! Part No.!! Plate Type !! Colonies !! Other Observations !! | ||
+ | |- | ||
+ | ! pSB 3K3 | ||
+ | | Kan || 10s of colonies || n/a || | ||
+ | |- | ||
+ | ! E0240 | ||
+ | | Amp || 100s of colonies || no color || | ||
+ | |- | ||
+ | ! J23101 | ||
+ | | Amp || 1000s of colonies || Pink in color. || | ||
+ | |- | ||
+ | ! J23113 | ||
+ | | Amp || 100s of colonies || n/a || | ||
+ | |- | ||
+ | ! J23116 | ||
+ | | Amp || 100s of colonies || n/a || | ||
+ | |- | ||
+ | ! J23102 | ||
+ | | Amp ||10s of colonies || Pink in color.|| | ||
+ | |- | ||
+ | ! R0040 | ||
+ | | Amp ||10s of colonies || n/a || | ||
+ | |} | ||
+ | |||
+ | *Group 2: Adam, Kevin and Maria Plates | ||
+ | *Observations on the Colonies | ||
+ | |||
+ | {| cellpadding="5" border="1" padding="5" | ||
+ | |+ | ||
+ | |- | ||
+ | ! Part No.!! Plate Type !! Colonies !! Other Observations !! | ||
+ | |- | ||
+ | ! R0011 | ||
+ | | Amp || 10s of colonies || n/a || | ||
+ | |- | ||
+ | ! J23100 | ||
+ | | Amp || 10s of colonies || Slightly pink || | ||
+ | |- | ||
+ | ! J23103 | ||
+ | | Amp || low 100s of colonies || Pink in color || | ||
+ | |- | ||
+ | ! J23104 | ||
+ | | Amp || 100s of colonies || n/a || | ||
+ | |- | ||
+ | ! J23105 | ||
+ | | Amp || 100s of colonies || n/a || | ||
+ | |- | ||
+ | ! J23107 | ||
+ | | Amp ||1000s of colonies || n/a || | ||
+ | |- | ||
+ | ! J23110 | ||
+ | | Amp ||1000s of colonies || n/a || | ||
+ | |} | ||
+ | |||
+ | *Problems: | ||
+ | **Part J23106 was taken from well 18D, when it actually is located in well 18O. | ||
+ | **Part Q04511 was actually taken from well 18D | ||
+ | **Part R0011 was plated twice. The plate with the most colonies was used for growth overnight. | ||
+ | |||
+ | [[User:MandM|MandM]] 22:52, 11 June 2009 (UTC) | ||
---- | ---- | ||
+ | |||
===Tasks=== | ===Tasks=== | ||
+ | * Literature Discussion | ||
+ | * Discussion of iGEM ideas | ||
---- | ---- | ||
- | |||
- | |||
====Wetlab==== | ====Wetlab==== | ||
- | * | + | * Picking Transformed Colonies to be grown overnight for Mini-preps (5mL) and two Maxi-preps(125mL) |
- | + | [[User:MandM|MandM]] 22:55, 11 June 2009 (UTC) |
Latest revision as of 15:48, 5 August 2009
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Contents |
Thursday 11 June 2009
Results
- Note these were plated Wednesday evening with two groups splitting the work.
- Group 1: Craig, Clay, Afton plates
- Observations on the Controls
Control | Plate Type | Observation | |
---|---|---|---|
NEB10β (+) | LB | lawn | |
NEB10β (-) | Cm | no growth | |
NEB10β (-) | Amp | no growth | |
NEB10β (-) | Kan | 3 colonies |
- Growth on Kan could have resulted from a non-homogeneous medium with very small sections of the plate being able to sustain life. Or there was degradation of the Kan-selective medium.
- Observations on the Colonies
Part No. | Plate Type | Colonies | Other Observations | |
---|---|---|---|---|
pSB 3K3 | Kan | 10s of colonies | n/a | |
E0240 | Amp | 100s of colonies | no color | |
J23101 | Amp | 1000s of colonies | Pink in color. | |
J23113 | Amp | 100s of colonies | n/a | |
J23116 | Amp | 100s of colonies | n/a | |
J23102 | Amp | 10s of colonies | Pink in color. | |
R0040 | Amp | 10s of colonies | n/a |
- Group 2: Adam, Kevin and Maria Plates
- Observations on the Colonies
Part No. | Plate Type | Colonies | Other Observations | |
---|---|---|---|---|
R0011 | Amp | 10s of colonies | n/a | |
J23100 | Amp | 10s of colonies | Slightly pink | |
J23103 | Amp | low 100s of colonies | Pink in color | |
J23104 | Amp | 100s of colonies | n/a | |
J23105 | Amp | 100s of colonies | n/a | |
J23107 | Amp | 1000s of colonies | n/a | |
J23110 | Amp | 1000s of colonies | n/a |
- Problems:
- Part J23106 was taken from well 18D, when it actually is located in well 18O.
- Part Q04511 was actually taken from well 18D
- Part R0011 was plated twice. The plate with the most colonies was used for growth overnight.
MandM 22:52, 11 June 2009 (UTC)
Tasks
- Literature Discussion
- Discussion of iGEM ideas
Wetlab
- Picking Transformed Colonies to be grown overnight for Mini-preps (5mL) and two Maxi-preps(125mL)
MandM 22:55, 11 June 2009 (UTC)