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From 2009.igem.org

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Revision as of 08:14, 7 October 2009

Notebook Notebook Home Protocol AND Gate 1 Input Core Bistable AND Gate 2 Output System Assembly Notes By Person (PDF)
	Protocol for PCR with EasyPfu DNA Polymerase download PDF version Preparation for Reaction Mixture Reagent Final concentration Volume 10X Taq buffer 1X 2 µl 2.5 mM dNTP mix 0.25 mM 2 µl Forward Primer 0.2-0.4 µM 1 µl Reverse Primer 0.2-0.4 µM 1 µl EasyPfu DNA Polymerase 25 units 1 µl Template DNA As required <0.5 µg ddH2O to final volume Cycling Conditions Step Temperature/℃ Time/min Initial Denaturation 94 5 Denaturation 94 0.5 Primer Annealing As required As required Extending 72 As required(0.5kb/min) Final Extending 72 10 Number of Cycles 32-35 Cooling Down 4 10 ^Top

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