The terminator colonies didn’t grow in the liquid LB medium.
We performed miniprep to get purified RBS biobrick (Protocol 2).
Taís
ColiGuard
Lux S (BBa_K091109) enzyme restriction
We tried one more time the enzyme restriction with XbaI and PstI, but didn’t find the part again. We expected 530 band size, but just was noticed plasmid band. Something is wrong with this part.