"
Team:EPF-Lausanne/Modeling
From 2009.igem.org
(Difference between revisions)
| Line 45: | Line 45: | ||
==Molecular dynamics: a little theory== | ==Molecular dynamics: a little theory== | ||
| + | <html> | ||
| + | <script type="text/javascript" language="JavaScript"> | ||
<p> | <p> | ||
<a href="javascript:ReverseDisplay('hs1')"> Click here to show/hide</a> | <a href="javascript:ReverseDisplay('hs1')"> Click here to show/hide</a> | ||
| Line 69: | Line 71: | ||
[[Media:Introduction_to_molecular_Dynamics_Simulation.pdf | Introduction to Molecular Dynamics Simulation - Michael P. Allen]] | [[Media:Introduction_to_molecular_Dynamics_Simulation.pdf | Introduction to Molecular Dynamics Simulation - Michael P. Allen]] | ||
| - | + | </p> | |
| + | </div> | ||
| + | </html> | ||
| + | <br> | ||
==Steps== | ==Steps== | ||
Revision as of 07:54, 21 September 2009
Contents |
Protein domain of interest
Our protein of interest is LOVTAP. This protein was sythetically engineered by [http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&cmd=search&term=18667691 Sosnick] group. It is a fusion protein between a LOV domain (Avena Sativa phototropin 1) and the E. Coli tryptophan repressor. This protein undergoes changes under light activation as shown by Sosnick et al, in fact when the protein is activated by light it binds DNA and inversely. For more information about LOVTAP protein please click here.
Goal
Starting material
Both LOV domain crystallography files were obtained from [http://www.rcsb.org/pdb/home/home.do RCSB]:
- [http://www.rcsb.org/pdb/explore/explore.do?structureId=2V0W Light activated LOV domain]
- [http://www.rcsb.org/pdb/explore/explore.do?structureId=2V0U Dark LOV domain]
These crystallographies were done by [http://www.ncbi.nlm.nih.gov/pubmed/18001137 Halavaty et al.].
Molecular dynamics: a little theory