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Team:Kyoto/CiC/Notebook/1012-1016
From 2009.igem.org
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===Thursday, 15 October=== | ===Thursday, 15 October=== | ||
====To Do==== | ====To Do==== | ||
| + | *Experiments to approach subgoalA | ||
====Results==== | ====Results==== | ||
| + | No NBD-PE signal was observed in HeLa cells. | ||
| + | This might be because we did not collect the right fraction after density gradient centrifugation and thus lost the liposomes. | ||
{{:Team:Kyoto/Pad_between_dates}} | {{:Team:Kyoto/Pad_between_dates}} | ||
Revision as of 18:44, 21 October 2009
- 0907-0911
- constructing HIV-TAT-(LALAAAA)3-generator
- 0914-0918
- constructing HIV-TAT-(LALAAAA)3-generator
- 0921-0925
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 0928-1002
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 1005-1009
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 1012-1016
- Observation
- 1019-1023
- Observation
1012-1018 : what to do. what to do. what to do. what to do.
Monday, 12 October
To Do
Results
Tuesday, 13 October
To Do
- sequence
- sig-GFP generator
- sig-EGFP generator(HeLa)
Results
- We assured that the plasmid include the correct sequence by analyzing it with sequencer.(091015)
Wednesday, 14 October
To Do
No experiments were undertaken
Thursday, 15 October
To Do
- Experiments to approach subgoalA
Results
No NBD-PE signal was observed in HeLa cells. This might be because we did not collect the right fraction after density gradient centrifugation and thus lost the liposomes.
Friday, 16 October
To Do
Results
Saturday, 17 October
To Do
Results
Saturday, 17 October
To Do
Results
Sunday, 18 October
To Do
Results
