Team:Kyoto/CiC/Notebook/0907-0911

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0907-0911
constructing HIV-TAT-(LALAAAA)3-generator
0914-0918
constructing HIV-TAT-(LALAAAA)3-generator
0921-0925
constructing sig-GFP generator,sig-EGFP generator(HeLa)
0928-1002
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1005-1009
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1012-1016
Observation
1019-1023
Observation

097-0911 : Plasmid Construction of HIV-TAT-(LALAAAA)3-generator

Wednesday, 16 September

To Do

  • HIV-TAT-(LALAAAA)3
    • PCR
    • Restriction Enzyme Digestion(EcoR1 + Spe1)
  • B0015
    • Restriction Enzyme Digestion(EcoR1 + Xba1)

Results

  • Band of expected size were observed.

Fryday, 11 September

To Do

  • HIV-TAT-(LALAAAA)3
    • Ligation
    • Transformation

Results

ligation
conc.
NO.Sample nameconc./(ng/ul)
1HIV-TAT sample1(EcoR1,Spe1)36.2
2HIV-TAT sample2(EcoR1,Spe1)21.7
3HIV-TAT sample3(EcoR1,Spe1)47.9
4B0015(EcoR1,Xba1)2.48
Transformation

Colony was observed (090912)　

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