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Team:Kyoto/CiC/Notebook/0914-0918
From 2009.igem.org
- 0907-0911
- constructing HIV-TAT-(LALAAAA)3-generator
- 0914-0918
- constructing HIV-TAT-(LALAAAA)3-generator
- 0921-0925
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 0928-1002
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 1005-1009
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 1012-1016
- Observation
- 1019-1023
- Observation
0914-0918 : constructing HIV-TAT-(LALAAAA)3-generator
Monday, 14 September
To Do
- No experiments were undertaken
Tuesday, 15 September
To Do
- HIV-TAT-(LALAAAA)3-generator
- Colony PCR
Results
Bands of expected size were observed.
Wednesday, 16 September
To Do
- HIV-TAT-(LALAAAA)3+B0015(terminator)
- miniprep
- Restriction Enzyme Digestion(Xba1,Pst1)
- ligation
- I712074(T7promoter)
- Restriction Enzyme Digestion(Spe1,Pst1)
- ligation
Results
Transformation
Colony was observed (090917)
Thursday, 17 September
To Do
- HIV-TAT-(LALAAAA)3-generator
- colony PCR
Results
- Binds of expected size were observed.
Friday, 18 September
To Do
- No experiments were undertaken
