Team:Kyoto/CiC/Notebook/1019-1023
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====To Do==== | ====To Do==== | ||
* subgoal A --- Confirmation of function of TAT-liposome | * subgoal A --- Confirmation of function of TAT-liposome | ||
+ | **observation by microscope | ||
* Subgoal B --- Liposomes with Mitochondrial Translocases | * Subgoal B --- Liposomes with Mitochondrial Translocases | ||
- | * Subgoal C --- sig- | + | **Mitochondoria isolation |
+ | **expressing sig-GFP in vitro with mitochondoria | ||
+ | * Subgoal C --- sig-EGFP expression in HeLa | ||
+ | **transfomation | ||
====Results==== | ====Results==== | ||
- | + | =====subgoal A --- Confirmation of function of TAT-liposome===== | |
+ | Liposome was taken by HeLa cells.See the [[Team:Kyoto/CiC/Results|results]]. | ||
- | === | + | =====Subgoal C --- sig-EGFP expression in HeLa===== |
- | + | sig-EGFP was made a localization in mitochondoria. | |
- | + | See the [[Team:Kyoto/CiC/Results|results]]. | |
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{{:Team:Kyoto/Pad_between_dates}} | {{:Team:Kyoto/Pad_between_dates}} | ||
- | === | + | ===Tuesday, 20 October=== |
====To Do==== | ====To Do==== | ||
+ | * Subgoal B --- Liposomes with Mitochondrial Translocases | ||
+ | **observation with microscope | ||
====Results==== | ====Results==== | ||
- | + | See the [[Team:Kyoto/CiC/Results|results]]. | |
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{{:Team:Kyoto/Pad_between_dates}} | {{:Team:Kyoto/Pad_between_dates}} | ||
- | === | + | ===Wednesday, 21 October=== |
- | + | ||
- | + | ||
- | + | ||
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- | + | ||
====To Do==== | ====To Do==== | ||
+ | * subgoal A --- Confirmation of function of TAT-liposome | ||
+ | **making liposome by natural swelling method and extruder | ||
+ | **add liposome to HeLa cells | ||
====Results==== | ====Results==== | ||
+ | According to the expectation,HIV-TAT-(LALAAAA)3-generator may work. | ||
+ | Liposome is taken by HeLa Cells. | ||
+ | See the [[Team:Kyoto/CiC/Results|results]]. | ||
{{:Team:Kyoto/Pad_endof_dates}} | {{:Team:Kyoto/Pad_endof_dates}} | ||
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Latest revision as of 03:08, 22 October 2009
- 0907-0911
- constructing HIV-TAT-(LALAAAA)3-generator
- 0914-0918
- constructing HIV-TAT-(LALAAAA)3-generator
- 0921-0925
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 0928-1002
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 1005-1009
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 1012-1016
- Observation
- 1019-1023
- Observation
1019-1025 :Obserbation
Monday, 19 October
To Do
- subgoal A --- Confirmation of function of TAT-liposome
- observation by microscope
- Subgoal B --- Liposomes with Mitochondrial Translocases
- Mitochondoria isolation
- expressing sig-GFP in vitro with mitochondoria
- Subgoal C --- sig-EGFP expression in HeLa
- transfomation
Results
subgoal A --- Confirmation of function of TAT-liposome
Liposome was taken by HeLa cells.See the results.
Subgoal C --- sig-EGFP expression in HeLa
sig-EGFP was made a localization in mitochondoria. See the results.
Tuesday, 20 October
To Do
- Subgoal B --- Liposomes with Mitochondrial Translocases
- observation with microscope
Results
See the results.
Wednesday, 21 October
To Do
- subgoal A --- Confirmation of function of TAT-liposome
- making liposome by natural swelling method and extruder
- add liposome to HeLa cells
Results
According to the expectation,HIV-TAT-(LALAAAA)3-generator may work. Liposome is taken by HeLa Cells. See the results.