Team:Kyoto/CiC/Notebook/1019-1023
From 2009.igem.org
(Difference between revisions)
(→0914-0918 : what to do. what to do. what to do. what to do.) |
(→0914-0918 : Plasmid Construction of HIV-TAT-(LALAAAA)3-generator) |
||
Line 81: | Line 81: | ||
**Colony PCR | **Colony PCR | ||
**Insert check | **Insert check | ||
+ | |||
+ | {{:Team:Kyoto/Pad_start_dates}} | ||
+ | ===Monday, 14 August=== | ||
+ | ====To Do==== | ||
+ | *p-2-(1)(4)(7)(8)(10) | ||
+ | **Miniprep | ||
+ | **Restriction Enzyme Digestion | ||
+ | *p-2-(1)(4) E.X | ||
+ | **PCR purification kit | ||
+ | *p-2-(1)(7)(8)(10) E.S | ||
+ | **Gel extraction | ||
+ | *4-(1)(2)(3) | ||
+ | **Colony PCR | ||
+ | **Insert check | ||
+ | |||
+ | ====Results==== | ||
+ | =====p-2-(1)(4)(7)(8)(10)===== | ||
+ | =====p-2-(1)(7)(8)(10) E.S===== | ||
+ | *Gel extraction kit | ||
+ | [[Image:Kyoto_0914_PCR.png|250px]] | ||
+ | {| class="table" | ||
+ | |+Miniprep product conc. | ||
+ | !NO.!!Sample name!!conc./(ng/ul) | ||
+ | |- | ||
+ | |1||p-2-(1)||9.3 | ||
+ | |- | ||
+ | |2||p-2-(7)||25.9 | ||
+ | |- | ||
+ | |3||p-2-(8)||8.6 | ||
+ | |- | ||
+ | |4||p-2-(10)||7.5 | ||
+ | |} | ||
+ | |||
+ | =====4-(1)(2)(3)===== | ||
+ | [[Image:Kyoto_0914_PCR_2.png|250px]] | ||
+ | *This result indicates that (1)a, (2)b and (3)a were inserted properly. | ||
+ | |||
+ | {{:Team:Kyoto/Pad_between_dates}} | ||
{{:Team:Kyoto/Pad_between_dates}} | {{:Team:Kyoto/Pad_between_dates}} |
Revision as of 09:31, 21 October 2009
- 0907-0911
- constructing HIV-TAT-(LALAAAA)3-generator
- 0914-0918
- constructing HIV-TAT-(LALAAAA)3-generator
- 0921-0925
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 0928-1002
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 1005-1009
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 1012-1016
- Observation
- 1019-1023
- Observation
0914-0918 : Plasmid Construction of HIV-TAT-(LALAAAA)3-generator
Monday, 14 August
To Do
- p-2-(1)(4)(7)(8)(10)
- Miniprep
- Restriction Enzyme Digestion
- p-2-(1)(4) E.X
- PCR purification kit
- p-2-(1)(7)(8)(10) E.S
- Gel extraction
- 4-(1)(2)(3)
- Colony PCR
- Insert check
Results
p-2-(1)(4)(7)(8)(10)
p-2-(1)(7)(8)(10) E.S
- Gel extraction kit
NO. | Sample name | conc./(ng/ul) |
---|---|---|
1 | p-2-(1) | 9.3 |
2 | p-2-(7) | 25.9 |
3 | p-2-(8) | 8.6 |
4 | p-2-(10) | 7.5 |
4-(1)(2)(3)
- This result indicates that (1)a, (2)b and (3)a were inserted properly.
Tuesday, 15 September
To Do
Further to 14 Sept.
- p-2-(1)(4)(7)(8)(10)
- Miniprep
- Restriction Enzyme Digestion
- p-2-(1)(4) E.X
- PCR purification kit
- p-2-(1)(7)(8)(10) E.S
- Gel extraction
- 4-(1)(2)(3)
- Colony PCR
- Insert check
Monday, 14 August
To Do
- p-2-(1)(4)(7)(8)(10)
- Miniprep
- Restriction Enzyme Digestion
- p-2-(1)(4) E.X
- PCR purification kit
- p-2-(1)(7)(8)(10) E.S
- Gel extraction
- 4-(1)(2)(3)
- Colony PCR
- Insert check
Results
p-2-(1)(4)(7)(8)(10)
p-2-(1)(7)(8)(10) E.S
- Gel extraction kit
NO. | Sample name | conc./(ng/ul) |
---|---|---|
1 | p-2-(1) | 9.3 |
2 | p-2-(7) | 25.9 |
3 | p-2-(8) | 8.6 |
4 | p-2-(10) | 7.5 |
4-(1)(2)(3)
- This result indicates that (1)a, (2)b and (3)a were inserted properly.
Wednesday, 16 September
To Do
- ligation
- transformation
Results
ligation
NO. | Sample name | conc./(ng/ul) |
---|---|---|
1 | 11(stu1) | 103.2 |
Transformation
No colony was observed (090917 10:00)
Thursday, 17 September
To Do
- Make repetitive sequence (MPR)
- Transformation(retry)